Inhibitors | Comment | Organism | Structure |
---|---|---|---|
4-chloromercuribenzoate | 0.1 mM, 2% residual activity | Streptomyces hygroscopicus | |
Ca2+ | 1 mM, 54% residual activity | Streptomyces hygroscopicus | |
EDTA | 0.05 mM, almost complete inactivation | Streptomyces hygroscopicus | |
Fe2+ | 1 mM, 50% residual activity | Streptomyces hygroscopicus | |
iodoacetic acid | 1 mM, 18% residual activity | Streptomyces hygroscopicus | |
Mg2+ | 1 mM, 44% residual activity | Streptomyces hygroscopicus | |
phosphate buffer | not inhibitory at 10 mM, 50% loss of activity in 100 mM phosphate buffer | Streptomyces hygroscopicus | |
Zn2+ | 1 mM, 24% residual activity | Streptomyces hygroscopicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.052 | - |
acetyl-CoA | pH 8.0, 30°C | Streptomyces hygroscopicus | |
0.13 | - |
oxaloacetic acid | pH 8.0, 30°C | Streptomyces hygroscopicus | |
0.385 | - |
3-(hydroxyphosphinoyl)pyruvate | pH 8.0, 30°C | Streptomyces hygroscopicus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
48000 | - |
2 * 48000, SDS-PAGE | Streptomyces hygroscopicus |
90000 | 98000 | gel fitlration | Streptomyces hygroscopicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + H2O + 3-(hydroxyphosphinoyl)pyruvate | Streptomyces hygroscopicus | - |
phosphinomethylmalate + CoA | - |
? | |
acetyl-CoA + H2O + 3-(hydroxyphosphinoyl)pyruvate | Streptomyces hygroscopicus SF-1293 | - |
phosphinomethylmalate + CoA | - |
? | |
acetyl-CoA + H2O + oxaloacetic acid | Streptomyces hygroscopicus | - |
? + CoA | - |
? | |
acetyl-CoA + H2O + oxaloacetic acid | Streptomyces hygroscopicus SF-1293 | - |
? + CoA | - |
? | |
additional information | Streptomyces hygroscopicus | no substrates: propionyl-CoA or butanoyl-CoA and alpha-oxoacids such as pyruvate, 2-oxoglutarate, deamino-2-oxodemethylphosphinotricin or phosphonopyruvate | ? | - |
? | |
additional information | Streptomyces hygroscopicus SF-1293 | no substrates: propionyl-CoA or butanoyl-CoA and alpha-oxoacids such as pyruvate, 2-oxoglutarate, deamino-2-oxodemethylphosphinotricin or phosphonopyruvate | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces hygroscopicus | Q9LCB4 | - |
- |
Streptomyces hygroscopicus SF-1293 | Q9LCB4 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Streptomyces hygroscopicus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
546.8 | - |
pH 8.0, 30°C | Streptomyces hygroscopicus |
Storage Stability | Organism |
---|---|
4°C, lyophilized powder, 5% loss of activity within 3 months | Streptomyces hygroscopicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + H2O + 3-(hydroxyphosphinoyl)pyruvate | - |
Streptomyces hygroscopicus | phosphinomethylmalate + CoA | - |
? | |
acetyl-CoA + H2O + 3-(hydroxyphosphinoyl)pyruvate | - |
Streptomyces hygroscopicus SF-1293 | phosphinomethylmalate + CoA | - |
? | |
acetyl-CoA + H2O + oxaloacetic acid | - |
Streptomyces hygroscopicus | ? + CoA | - |
? | |
acetyl-CoA + H2O + oxaloacetic acid | - |
Streptomyces hygroscopicus SF-1293 | ? + CoA | - |
? | |
additional information | no substrates: propionyl-CoA or butanoyl-CoA and alpha-oxoacids such as pyruvate, 2-oxoglutarate, deamino-2-oxodemethylphosphinotricin or phosphonopyruvate | Streptomyces hygroscopicus | ? | - |
? | |
additional information | no substrates: propionyl-CoA or butanoyl-CoA and alpha-oxoacids such as pyruvate, 2-oxoglutarate, deamino-2-oxodemethylphosphinotricin or phosphonopyruvate | Streptomyces hygroscopicus SF-1293 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 48000, SDS-PAGE | Streptomyces hygroscopicus |
Synonyms | Comment | Organism |
---|---|---|
2-phosphinomethylmalic acid synthase | - |
Streptomyces hygroscopicus |
pmmS | - |
Streptomyces hygroscopicus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
- |
Streptomyces hygroscopicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Streptomyces hygroscopicus |