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Literature summary for 2.3.3.21 extracted from
- Isoleucine biosynthesis in Leptospira interrogans serotype lai strain 56601 proceeds via a threonine-independent pathway (2004), J. Bacteriol., 186, 5400-5409.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Leptospira interrogans |
| gene cimA, DNA and amino acid sequence determination and analysis, expression as His6-tagged enzyme in Escherichia coli | Leptospira interrogans |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| isoleucine | - |
Leptospira interrogans |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.04 | - |
pyruvate | recombinant enzyme, pH 8.0, 37°C | Leptospira interrogans | |
| 0.043 | - |
pyruvate | - |
Leptospira interrogans | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 60000 | - |
x * 60000, recombinant His6-tagged enzyme, SDS-PAGE | Leptospira interrogans |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acetyl-CoA + pyruvate + H2O | Leptospira interrogans | the enzyme is involved in the threonine-independent biosynthesis of isoleucine via an alternative beta-methyl-D-malate pathway, the expression of cimA is transcriptionally regulated by isoleucine | CoA + (R)-citramalate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Leptospira interrogans | - |
serotype lai strain 56601 | - |
| Leptospira interrogans | - |
serotype lai strain 56601, gene cimA or LA2350 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Leptospira interrogans |
| recombinant His6-tagged enzyme 14.3fold from Escherichia coli by nickel affinity chromatography to homogeneity | Leptospira interrogans |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.174 | - |
crude extract of Escherichia coli recombinantly expressing CimA | Leptospira interrogans |
| 2.5 | - |
- |
Leptospira interrogans |
| 2.53 | - |
purified recombinant His6-tagged enzyme | Leptospira interrogans |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acetyl-CoA + pyruvate | the enzyme is strictly specific for pyruvate as keto acid substrate | Leptospira interrogans | (3S)-citramalyl-CoA | - |
? | |
| acetyl-CoA + pyruvate + H2O | the enzyme is involved in the threonine-independent biosynthesis of isoleucine via an alternative beta-methyl-D-malate pathway, the expression of cimA is transcriptionally regulated by isoleucine | Leptospira interrogans | CoA + (R)-citramalate | - |
? | |
| acetyl-CoA + pyruvate + H2O | the enzyme is strictly specific for pyruvate as the keto acid substrate | Leptospira interrogans | CoA + (R)-citramalate | - |
? | |
| additional information | LA2350 is a citramalate synthase without detectable alpha-isopropylmalate synthase activity | Leptospira interrogans | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 60000, recombinant His6-tagged enzyme, SDS-PAGE | Leptospira interrogans |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| CimA | - |
Leptospira interrogans |
| citramalate synthase | - |
Leptospira interrogans |
| LA2350 | - |
Leptospira interrogans |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
- |
Leptospira interrogans |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| additional information | - |
temperature profile, overview | Leptospira interrogans |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
- |
40 | 80% of maximal activity within this range | Leptospira interrogans |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 2.41 | - |
pyruvate | - |
Leptospira interrogans | |
| 2.41 | - |
pyruvate | recombinant enzyme, pH 8.0, 37°C | Leptospira interrogans | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
assay at | Leptospira interrogans |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| acetyl-CoA | - |
Leptospira interrogans | |
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Release 2023.1 (January 2023)
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