Any feedback?
Literature summary for 2.3.3.21 extracted from
- (R)-citramalate synthase in methanogenic archaea (1999), J. Bacteriol., 181, 331-333.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene MJ1392 or cimA, DNA and amino acid sequence determination and analysis, functional overexpression in Escherichia coli strain BL21(DE3) | Methanocaldococcus jannaschii |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acetyl-CoA + pyruvate + H2O | Methanocaldococcus jannaschii | the enzyme is involved in the biosynthesis of isoleucine | CoA + (2R)-2-hydroxy-2-methylbutanedioate | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Methanocaldococcus jannaschii | - |
gene MJ1392 or cimA | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant CimA 26.5fold from Escherichia coli strain BL21(DE3) by ammonium sulfate fractionation and heat treatment at 60°C for 10 min, followed by gel filtration | Methanocaldococcus jannaschii |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 2.87 | - |
purified recombinant enzyme | Methanocaldococcus jannaschii |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acetyl-CoA + pyruvate + H2O | - |
Methanocaldococcus jannaschii | CoA + (2R)-2-hydroxy-2-methylbutanedioate | (R)-citramalate is 2-methylmalate | ? | |
| acetyl-CoA + pyruvate + H2O | the enzyme is involved in the biosynthesis of isoleucine | Methanocaldococcus jannaschii | CoA + (2R)-2-hydroxy-2-methylbutanedioate | - |
? | |
| additional information | no activity with alpha-ketoglutarate, alpha-ketoadipate, alpha-ketopimelate, alpha-ketoisovalerate, and acetyl-CoA | Methanocaldococcus jannaschii | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| CimA | - |
Methanocaldococcus jannaschii |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
assay at | Methanocaldococcus jannaschii |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
assay at | Methanocaldococcus jannaschii |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| acetyl-CoA | - |
Methanocaldococcus jannaschii | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
