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Literature summary for 2.3.3.21 extracted from
- Enzymes of the citramalate cycle in Rhodospirillum rubrum (2009), Microbiology, 78, 16-24.
No PubMed abstract available
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 3.2 | - |
acetyl-CoA | measured by CoA formation | Rhodospirillum rubrum |  |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | only 30% activity compared to Mn2+ presence | Rhodospirillum rubrum | |
| Mn2+ | reaction depends on the presence of Mn2+ | Rhodospirillum rubrum | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acetyl-CoA + pyruvate + H2O | Rhodospirillum rubrum | 14C-labeled acetyl-CoA | CoA + (2R)-2-hydroxy-2-methylbutanedioate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rhodospirillum rubrum | - |
- |
- |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.016 | - |
pyruvate consumption measured, Rhodospirillum rubrum cells grown in medium with acetate and NaHCO3 | Rhodospirillum rubrum |
| 0.017 | - |
pyruvate consumption measured, Rhodospirillum rubrum cells grown in medium with malate | Rhodospirillum rubrum |
| 0.032 | - |
CoA formation measured, Rhodospirillum rubrum cells grown in medium with malate | Rhodospirillum rubrum |
| 0.058 | - |
CoA formation measured, Rhodospirillum rubrum cells grown in medium with acetate and NaHCO3 | Rhodospirillum rubrum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acetyl-CoA + pyruvate + H2O | 14C-labeled acetyl-CoA | Rhodospirillum rubrum | CoA + (2R)-2-hydroxy-2-methylbutanedioate | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| citramalate synthase | - |
Rhodospirillum rubrum |
General Information
| General Information | Comment | Organism |
|---|---|---|
| metabolism | acetate assimilation via the citramalate cycle | Rhodospirillum rubrum |
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Release 2023.1 (January 2023)
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