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Literature summary for 2.3.3.8 extracted from

  • Kanao, T.; Fukui, T.; Atomi, H.; Imanaka, T.
    Kinetic and biochemical analyses on the reaction mechanism of a bacterial ATP-citrate lyase (2002), Eur. J. Biochem., 269, 3409-3416.
    View publication on PubMed
Search for more literature for 2.3.3.8

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Chlorobium limicola

Protein Variants

Protein Variants Comment Organism
H274A mutation abolishes both the catalytic activity and phosphorylation of the enzyme by ATP Chlorobium limicola

Inhibitors

Inhibitors Comment Organism Structure
ADP competitive inhibitor of ATP Chlorobium limicola

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information a strong negative cooperativity is observed with respect to citrate binding Chlorobium limicola
0.21
-
 ATP
-
 Chlorobium limicola

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + citrate + CoA Chlorobium limicola enzyme regulates both the direction and carbon flux of the carbon dioxide-fixing reductive tricarboxylic acid cycle ADP + phosphate + acetyl-CoA + oxaloacetate
-
 ir

Organism

Organism UniProt Comment Textmining
Chlorobium limicola
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme Chlorobium limicola

Reaction

Reaction Comment Organism Reaction ID
ADP + phosphate + acetyl-CoA + oxaloacetate = ATP + citrate + CoA ordered mechanism where the phosphate group of ATP is first covalently bound to the His273 catalytic residue, and subsequently transferred to the citrate molecule to form citryl phosphate Chlorobium limicola
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + citrate + CoA the first step of the reaction is reversible Chlorobium limicola ADP + phosphate + acetyl-CoA + oxaloacetate
-
 ir
ATP + citrate + CoA enzyme regulates both the direction and carbon flux of the carbon dioxide-fixing reductive tricarboxylic acid cycle Chlorobium limicola ADP + phosphate + acetyl-CoA + oxaloacetate
-
 ir
dATP + citrate + CoA 40% of the activity with ATP Chlorobium limicola dADP + phosphate + acetyl-CoA + oxaloacetate
-
 ?

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.037
-
 ADP
-
 Chlorobium limicola