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Literature summary for 2.4.1.1 extracted from

  • Tanabe, S.; Kobayashi, M.; Matsuda, K.
    Yeast glycogen phosphorylase: characterization of the dimeric form and its activation (1987), Agric. Biol. Chem., 51, 2465-2471.
No PubMed abstract available
Search for more literature for 2.4.1.1

Activating Compound

Activating Compound Comment Organism Structure
phosphate activation Saccharomyces cerevisiae
phosphate phosphorylation site: a single Thr-residue in the N-terminal region Saccharomyces cerevisiae
Phosphorylase kinase activation Saccharomyces cerevisiae

Inhibitors

Inhibitors Comment Organism Structure
AMP 10 mM, 55% inhibition, competitive to glucose 1-phosphate Saccharomyces cerevisiae
Caffeine weak Saccharomyces cerevisiae
Cu2+ 4 mM, more than 50% inhibition Saccharomyces cerevisiae
cyclodextrin alpha- or beta-, not gamma-cyclodextrin; i.e. structure analogue of alpha-1,4-linked starch molecule Saccharomyces cerevisiae
glucose 6-phosphate 5 mM, 54% inhibition Saccharomyces cerevisiae
Hg2+ 4 mM, more than 50% inhibition Saccharomyces cerevisiae
additional information not inhibited by IMP Saccharomyces cerevisiae
NaF 200 mM, complete inhibition Saccharomyces cerevisiae
UDPglucose 5 mM, 42% inhibition Saccharomyces cerevisiae
Zn2+ 4 mM, more than 50% inhibition Saccharomyces cerevisiae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information
-
 Saccharomyces cerevisiae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
100000
-
 2 * 100000, SDS-PAGE Saccharomyces cerevisiae

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
side-chain modification activation by phosphorylation Saccharomyces cerevisiae
side-chain modification phosphorylation site: a single Thr-residue in the N-terminal region Saccharomyces cerevisiae

Purification (Commentary)

Purification (Comment) Organism
Streptomycin, 33% ethanol, DEAE-Sephadex, glycogen-Sepharose Saccharomyces cerevisiae

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
42.3
-
-
 Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
glycogen + glucose 1-phosphate
-
 Saccharomyces cerevisiae glycogen + phosphate
-
 r
[(1->4)-alpha-D-glucosyl]n + phosphate
-
 Saccharomyces cerevisiae [(1->4)-alpha-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate
-
 r

Subunits

Subunits Comment Organism
dimer 2 * 100000, SDS-PAGE Saccharomyces cerevisiae

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
-
 Saccharomyces cerevisiae

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
25
-
 stable below Saccharomyces cerevisiae
40 50 inactivation Saccharomyces cerevisiae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.8
-
-
 Saccharomyces cerevisiae

pH Stability

pH Stability pH Stability Maximum Comment Organism
5 8 stable Saccharomyces cerevisiae

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
9.3
-
 AMP
-
 Saccharomyces cerevisiae