KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetic data | Typha latifolia | |
0.12 | - |
maltoheptaose | synthesis, isoenzyme P-2 | Typha latifolia | |
0.18 | - |
maltoheptaose | synthesis, isoenzyme P-2 | Typha latifolia | |
0.26 | - |
maltoheptaose | synthesis, isoenzyme P-1 | Typha latifolia | |
0.49 | - |
maltopentaose | synthesis, isoenzyme P-2 | Typha latifolia | |
0.59 | - |
maltoheptaose | synthesis, isoenzyme P-1 | Typha latifolia | |
0.88 | - |
maltotriose | synthesis, isoenzyme P-2 | Typha latifolia | |
1.1 | - |
maltoheptaose | synthesis, isoenzyme P-1 | Typha latifolia | |
1.7 | - |
maltopentaose | synthesis, isoenzyme P-1 | Typha latifolia |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
112000 | - |
x * 112000, SDS-PAGE | Typha latifolia |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Typha latifolia | - |
- |
- |
Purification (Comment) | Organism |
---|---|
isozymes P-1 and P-2, ammonium sulfate, DE-52, amylose-Sepharose, BioGel | Typha latifolia |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
pollen grain | mature | Typha latifolia | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
11 | - |
isozyme P-2 | Typha latifolia |
42.3 | - |
isozyme P-1 | Typha latifolia |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
amylose + glucose 1-phosphate | - |
Typha latifolia | amylose + phosphate | - |
r | |
maltoheptaose + glucose 1-phosphate | isozyme I | Typha latifolia | maltooctaose + phosphate | - |
r | |
maltopentaose + glucose 1-phosphate | isozyme I | Typha latifolia | maltohexaose + phosphate | - |
r | |
maltotriose + alpha-D-glucose 1-phosphate | - |
Typha latifolia | maltotetraose + phosphate | - |
r | |
starch + glucose 1-phosphate | soluble starch | Typha latifolia | starch + phosphate | - |
r | |
starch + glucose 1-phosphate | glucosylation of native starch granules occurs at 82%, isozyme P-1, or 11.5%, isozyme P-2, the rate of soluble starch, phosphorolysis at the same rate as soluble starch | Typha latifolia | starch + phosphate | - |
r | |
[(1->4)-alpha-D-glucosyl]n + phosphate | - |
Typha latifolia | [(1->4)-alpha-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate | - |
r |
Subunits | Comment | Organism |
---|---|---|
? | x * 112000, SDS-PAGE | Typha latifolia |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.5 | 5.8 | isoenzymes P-1 and P-2, phosphorolysis | Typha latifolia |
7.3 | 8 | synthesis | Typha latifolia |