General Stability | Organism |
---|---|
glycerol, 10%, stabilizes during purification | Zea mays |
sucrose, 10%, stabilizes during purification | Zea mays |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2,3-diphosphoglycerate | 5 mM, 30% inhibition | Zea mays | |
ADPglucose | 5 mM, 30% inhibition | Zea mays | |
additional information | not inhibited by fructose 6-phosphate; not inhibited by GMP; not inhibited by GTP; not inhibited by UMP, CMP, CTP | Zea mays |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetic data | Zea mays |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
150000 | - |
sucrose density gradient ultracentrifugation | Zea mays |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Zea mays | - |
sweet corn, var. Golden Bantam, Iowa Belle 104 | - |
Purification (Comment) | Organism |
---|---|
ammonium sulfate, DEAE-cellulose, hydroxylapatite | Zea mays |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
35 | 49 | - |
Zea mays |
49 | - |
- |
Zea mays |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(maltodextrin)n-1 + glucose 1-phosphate | - |
Zea mays | (maltodextrin)n + phosphate | - |
r | |
amylopectin + glucose 1-phosphate | best substrate | Zea mays | amylopectin + phosphate | - |
? | |
glycogen + glucose 1-phosphate | poor substrate | Zea mays | glycogen + phosphate | - |
? | |
maltoheptaose + glucose 1-phosphate | - |
Zea mays | maltooctaose + phosphate | - |
r | |
maltohexaose + glucose 1-phosphate | - |
Zea mays | maltoheptaose + phosphate | - |
r | |
maltooctaose + glucose 1-phosphate | - |
Zea mays | maltononaose + phosphate | - |
r | |
maltopentaose + glucose 1-phosphate | - |
Zea mays | maltohexaose + phosphate | - |
r | |
maltotetraose + glucose 1-phosphate | - |
Zea mays | maltopentaose + phosphate | - |
ir | |
maltotriose + glucose 1-phosphate | - |
Zea mays | maltotetraose + phosphate | - |
ir | |
[(1->4)-alpha-D-glucosyl]n + phosphate | polyglucose primer required | Zea mays | [(1->4)-alpha-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate | - |
r |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.8 | - |
shoulder at pH 6.5 | Zea mays |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | requirement | Zea mays | |
pyridoxal 5'-phosphate | 1 mol/mol subunit | Zea mays |