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Literature summary for 2.4.1.1 extracted from

  • Lee, E.Y.C.; Braun, J.J.
    Sweet corn phosphorylase: purification and properties (1973), Arch. Biochem. Biophys., 156, 276-286.
    View publication on PubMed

General Stability

General Stability Organism
glycerol, 10%, stabilizes during purification Zea mays
sucrose, 10%, stabilizes during purification Zea mays

Inhibitors

Inhibitors Comment Organism Structure
2,3-diphosphoglycerate 5 mM, 30% inhibition Zea mays
ADPglucose 5 mM, 30% inhibition Zea mays
additional information not inhibited by fructose 6-phosphate; not inhibited by GMP; not inhibited by GTP; not inhibited by UMP, CMP, CTP Zea mays

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information kinetic data Zea mays

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
150000
-
sucrose density gradient ultracentrifugation Zea mays

Organism

Organism UniProt Comment Textmining
Zea mays
-
sweet corn, var. Golden Bantam, Iowa Belle 104
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate, DEAE-cellulose, hydroxylapatite Zea mays

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
35 49
-
Zea mays
49
-
-
Zea mays

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(maltodextrin)n-1 + glucose 1-phosphate
-
Zea mays (maltodextrin)n + phosphate
-
r
amylopectin + glucose 1-phosphate best substrate Zea mays amylopectin + phosphate
-
?
glycogen + glucose 1-phosphate poor substrate Zea mays glycogen + phosphate
-
?
maltoheptaose + glucose 1-phosphate
-
Zea mays maltooctaose + phosphate
-
r
maltohexaose + glucose 1-phosphate
-
Zea mays maltoheptaose + phosphate
-
r
maltooctaose + glucose 1-phosphate
-
Zea mays maltononaose + phosphate
-
r
maltopentaose + glucose 1-phosphate
-
Zea mays maltohexaose + phosphate
-
r
maltotetraose + glucose 1-phosphate
-
Zea mays maltopentaose + phosphate
-
ir
maltotriose + glucose 1-phosphate
-
Zea mays maltotetraose + phosphate
-
ir
[(1->4)-alpha-D-glucosyl]n + phosphate polyglucose primer required Zea mays [(1->4)-alpha-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate
-
r

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.8
-
shoulder at pH 6.5 Zea mays

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate requirement Zea mays
pyridoxal 5'-phosphate 1 mol/mol subunit Zea mays