Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.4.1.1 extracted from

  • Shivaram, K.N.
    Purification and properties of potato phosphorylase isozymes (1976), Z. Naturforsch. C, 31, 424-432.
No PubMed abstract available

Activating Compound

Activating Compound Comment Organism Structure
2-mercaptoethanol slight activation Solanum tuberosum
cysteine activation Solanum tuberosum
cysteine slight Solanum tuberosum
glutathione slight stimulation Solanum tuberosum
additional information not activated by indoleacetic acid and gibberellic acid Solanum tuberosum

Inhibitors

Inhibitors Comment Organism Structure
Ag+ 1 mM, complete inhibition Solanum tuberosum
Fe2+ 1 mM, approx. 40% inhibition of synthesis Solanum tuberosum
Hg2+ 1 mM, complete inhibition Solanum tuberosum
additional information not inhibited by 2-mercaptoethanol; not inhibited by agarose; not inhibited by gibberellic acid, indolyl-3-acetic acid; not inhibited by Ni2+ Solanum tuberosum
p-chloromercuribenzoate
-
Solanum tuberosum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information kinetic data Solanum tuberosum
0.5
-
starch fast isoenzyme, starch phosphorolysis Solanum tuberosum
0.8
-
starch slow isoenzyme, starch phosphorolysis Solanum tuberosum
1.1
-
amylose fast isoenzyme, amylose phosphorolysis Solanum tuberosum
1.3
-
glycogen fast isoenzyme, glycogen phosphorolysis Solanum tuberosum
3.1
-
amylose slow isoenzyme, amylose phosphorolysis Solanum tuberosum
6.5
-
glycogen slow isoenzyme, glycogen phosphorolysis Solanum tuberosum
6.7
-
starch slow isoenzyme, starch synthesis Solanum tuberosum
8
-
starch fast isoenzyme, starch synthesis Solanum tuberosum
14.3
-
amylose slow isoenzyme, amylose synthesis Solanum tuberosum
20
-
amylose fast isoenzyme, amylose synthesis Solanum tuberosum
22.2
-
glycogen slow isoenzyme, glycogen synthesis Solanum tuberosum
40
-
glycogen fast isoenzyme, glycogen synthesis Solanum tuberosum

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ slight activation Solanum tuberosum
Mg2+ activation Solanum tuberosum
Mn2+ activation Solanum tuberosum
Mn2+ slight Solanum tuberosum
additional information not activated by Cu2+ Solanum tuberosum
additional information not activated by Zn2+ Solanum tuberosum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
40000
-
x * 40000, fast isozyme, SDS-PAGE Solanum tuberosum
104000
-
x * 104000, slow isozyme, SDS-PAGE Solanum tuberosum
165000
-
fast isozyme, gel filtration Solanum tuberosum

Organism

Organism UniProt Comment Textmining
Solanum tuberosum
-
cv. Adelheid or Rosa
-

Purification (Commentary)

Purification (Comment) Organism
2 isozymes: termed slow and fast, heat treatment, ammonium sulfate, DEAE-cellulose, preparative gel electrophoresis Solanum tuberosum

Source Tissue

Source Tissue Comment Organism Textmining
tuber
-
Solanum tuberosum
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.61
-
fast isozyme Solanum tuberosum
2.67
-
slow isozyme Solanum tuberosum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
amylose + glucose 1-phosphate
-
Solanum tuberosum amylose + phosphate
-
?
glycogen + glucose 1-phosphate
-
Solanum tuberosum glycogen + phosphate
-
?
starch + glucose 1-phosphate best substrate Solanum tuberosum starch + phosphate
-
r
starch + glucose 1-phosphate soluble starch Solanum tuberosum starch + phosphate
-
r
[(1->4)-alpha-D-glucosyl]n + phosphate
-
Solanum tuberosum [(1->4)-alpha-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate
-
r

Subunits

Subunits Comment Organism
? x * 40000, fast isozyme, SDS-PAGE Solanum tuberosum
? x * 104000, slow isozyme, SDS-PAGE Solanum tuberosum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30 35 slow isozyme Solanum tuberosum
35
-
fast isozyme Solanum tuberosum

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
55
-
unprimed phosphorylase II, 80% loss of activity after 10 min, albumin protects Solanum tuberosum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
additional information
-
pI: 5.0, slow isozyme Solanum tuberosum
additional information
-
pI: 5.5, fast isozyme Solanum tuberosum
5.2 5.6
-
Solanum tuberosum

pH Range

pH Minimum pH Maximum Comment Organism
4 8 approx. half-maximal activity at pH 4 and pH 8, slow isozyme Solanum tuberosum
4.2 7.6 approx. half-maximal activity at pH 4.2 and 7.6, fast isozyme Solanum tuberosum