Activating Compound | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | slight activation | Oryza sativa | |
DTT | slight activation | Oryza sativa | |
glutathione | slight activation | Oryza sativa | |
L-cysteine | slight activation | Oryza sativa |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ADP-glucose | strong inhibition | Oryza sativa | |
AMP | slight inhibition | Oryza sativa | |
Cyclodextrins | - |
Oryza sativa | |
D-fructose 6-phosphate | strong inhibition | Oryza sativa | |
D-mannose 1-phosphate | strong inhibition | Oryza sativa | |
IMP | slight inhibition | Oryza sativa | |
additional information | no or poor inhibition by ribose 5-phosphate, glucose 6-phosphate, fructose-1,6-bisphosphate, ADP and UDP | Oryza sativa | |
UDP-glucose | strong inhibition | Oryza sativa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0046 | - |
amylopectin | pH 5.5, 37°C, synthesis reaction | Oryza sativa | |
0.0106 | - |
soluble starch | pH 5.5, 37°C, synthesis reaction | Oryza sativa | |
0.0138 | - |
glycogen | pH 5.5, 37°C, synthesis reaction | Oryza sativa | |
0.0261 | - |
soluble starch | pH 5.5, 37°C, synthesis reaction | Oryza sativa | |
0.0418 | - |
Dextrin | pH 5.5, 37°C, synthesis reaction | Oryza sativa | |
2.1 | - |
alpha-D-glucose 1-phosphate | pH 5.5, 37°C, synthesis reaction | Oryza sativa |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Oryza sativa | 5829 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | not affected by Na+, K+, Mg2+, Ca2+ | Oryza sativa |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(1,4-alpha-D-glucosyl)n + phosphate | Oryza sativa | polysaccharide substrate is starch, phosphorolysis is the physiologic reaction of the starch degrading enzyme | (1,4-alpha-D-glucosyl)n-1 + alpha-D-glucose 1-phosphate | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Oryza sativa | - |
cv. Tainong 67, enzyme Pho1 | - |
Purification (Comment) | Organism |
---|---|
from seedlings, 299fold to homogeneity by protamine sulfate and ammonium sulfate fractionation, anion exchange chromatography, and dextrin affinity chromatography | Oryza sativa |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
seedling | etiolated, low enzyme content | Oryza sativa | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
substrate specificity | Oryza sativa |
1.8 | - |
purified enzyme | Oryza sativa |
Storage Stability | Organism |
---|---|
purified enzyme in 50 mM imidazole, pH 7.0, 1 mM EDTA, 1 mM DTT, at 4°C, at least 2 weeks stable | Oryza sativa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(1,4-alpha-D-glucosyl)n + phosphate | polysaccharide substrate is starch, phosphorolysis is the physiologic reaction of the starch degrading enzyme | Oryza sativa | (1,4-alpha-D-glucosyl)n-1 + alpha-D-glucose 1-phosphate | - |
r | |
(1,4-alpha-D-glucosyl)n + phosphate | polysaccharide substrates are soluble starch and Zulkowsky soluble starch, amylopectin, glycogen, and dextrin, amyylopectin is the best substrate | Oryza sativa | (1,4-alpha-D-glucosyl)n-1 + alpha-D-glucose 1-phosphate | - |
r | |
amylopectin + phosphate | - |
Oryza sativa | amylopectin + D-glucose-1-phosphate | - |
? | |
dextrin + alpha-D-glucose 1-phosphate | - |
Oryza sativa | dextrin + phosphate | - |
? | |
glycogen + phosphate | - |
Oryza sativa | alpha-D-glucan + alpha-D-glucose 1-phosphate | - |
r | |
maltodecaose + alpha-D-glucose 1-phosphate | low activity | Oryza sativa | ? + phosphate | - |
r | |
maltoheptaose + alpha-D-glucose 1-phosphate | low activity | Oryza sativa | maltooctaose + phosphate | - |
r | |
maltohexaose + alpha-D-glucose 1-phosphate | low activity | Oryza sativa | maltoheptaose + phosphate | - |
r | |
maltononaose + alpha-D-glucose 1-phosphate | low activity | Oryza sativa | maltodecaose + phosphate | - |
r | |
maltooctaose + alpha-D-glucose 1-phosphate | low activity | Oryza sativa | maltononaose + phosphate | - |
r | |
maltopentaose + alpha-D-glucose 1-phosphate | - |
Oryza sativa | maltohexaose + phosphate | - |
r | |
maltotetraose + alpha-D-glucose 1-phosphate | - |
Oryza sativa | maltopentaose + phosphate | - |
r | |
additional information | substrate specificity, no or nearly no activity with glucose, maltose, and maltotriose, overview | Oryza sativa | ? | - |
? | |
soluble starch + phosphate | - |
Oryza sativa | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
alpha-glucan phosphorylase | - |
Oryza sativa |
PHO | - |
Oryza sativa |
SP | - |
Oryza sativa |
starch phosphorylase | - |
Oryza sativa |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | 50 | - |
Oryza sativa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | - |
synthesis reaction | Oryza sativa |
6 | - |
assay at, phosphorolysis reaction | Oryza sativa |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
7 | - |
most stable at | Oryza sativa |