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Literature summary for 2.4.1.10 extracted from

  • Euzenat, O.; Guibert, A.; Combes, D.
    Production and purification of Bacillus subtilis C4 levansucrase: kinetic characterization of the enzyme (1998), Ann. N. Y. Acad. Sci., 864, 288-294.
No PubMed abstract available
Search for more literature for 2.4.1.10

Activating Compound

Activating Compound Comment Organism Structure
fructose slight activation Bacillus subtilis

Inhibitors

Inhibitors Comment Organism Structure
D-glucose
-
 Bacillus subtilis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
13.5
-
 sucrose at 37°C Bacillus subtilis

Organism

Organism UniProt Comment Textmining
Bacillus subtilis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
process optimization for large scale production Bacillus subtilis

Reaction

Reaction Comment Organism Reaction ID
sucrose + [6)-beta-D-fructofuranosyl-(2->]n alpha-D-glucopyranoside = D-glucose + [6)-beta-D-fructofuranosyl-(2->]n+1 alpha-D-glucopyranoside reaction kinetics are dependent on sucrose concentration Bacillus subtilis
a

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
195.6
-
 purified enzyme Bacillus subtilis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
sucrose + (2,6-beta-D-fructosyl)n reaction kinetics are dependent on sucrose concentration Bacillus subtilis glucose + (2,6-beta-D-fructosyl)n+1
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5 7 hydrolysis rate increases beyond pH 5.0 Bacillus subtilis