Literature summary for 2.4.1.10 extracted from
Jang, K.H.; Song, K.B.; Kim, C.H.; Chung, B.H.; Kang, S.A.; Chun, U.H.; Choue, R.W.; Rhee, S.K.
Comparison of characteristics of levan produced by different preparations of levansucrase from Zymomonas mobilis (2001), Biotechnol. Lett., 23, 339-344.
No PubMed abstract available
Application
Application |
Comment |
Organism |
synthesis |
immobilization of recombinant free levansucrase on magnetite leads to production of low molecular weight levan, increased thermal stability of the enzyme |
Zymomonas mobilis |
Cloned(Commentary)
Cloned (Comment) |
Organism |
expression as His-tagged protein in Escherichia coli BL21(DE3), genel evU |
Zymomonas mobilis |
Natural Substrates/ Products (Substrates)
Natural Substrates |
Organism |
Comment (Nat. Sub.) |
Natural Products |
Comment (Nat. Pro.) |
Rev. |
Reac. |
additional information |
Zymomonas mobilis |
production of low molecular weight levan in permeabilized recombinant E. coli cells |
? |
- |
? |
|
Organism
Organism |
UniProt |
Comment |
Textmining |
Zymomonas mobilis |
Q60114 |
gene levU |
- |
Purification (Commentary)
Purification (Comment) |
Organism |
recombinant from E. coli as His-tagged protein |
Zymomonas mobilis |
Substrates and Products (Substrate)
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
additional information |
production of low molecular weight levan in permeabilized recombinant E. coli cells |
Zymomonas mobilis |
? |
- |
? |
|
sucrose + (2,6-beta-D-fructosyl)n |
- |
Zymomonas mobilis |
glucose + (2,6-beta-D-fructosyl)n+1 |
molecular weight of produced levan is dependent on preparation method, immobilized recombinant enzyme and recombinant enzyme in permeabilized E. coli cells produces low molecular weight levan, free recombinant enzyme produces high molecular weight levan |
? |
|
Temperature Optimum [°C]
Temperature Optimum [°C] |
Temperature Optimum Maximum [°C] |
Comment |
Organism |
additional information |
- |
at lower temperatures, such as 4°C and 15°C, the transfructosylation is preferentially catalyzed rather than the hydrolysis of sucrose, but inversely at higher temperatures such as 30°C and 40°C the hydrolysis is preferentially catalyzed |
Zymomonas mobilis |