Any feedback?
Literature summary for 2.4.1.10 extracted from
- Catalytic biosynthesis of levan and short-chain fructooligosaccharides from sucrose-containing feedstocks by employing the levansucrase from Leuconostoc mesenteroides MTCC10508 (2019), Int. J. Biol. Macromol., 127, 486-495 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| truncated levansucrase gene lacking the signal peptide is expressed in Escherichia coli BL21 cells | Leuconostoc mesenteroides |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| EDTA | the presence of 0.05 mM EDTA results in a decrease in the enzymatic activity by about 80% | Leuconostoc mesenteroides |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 131 | - |
sucrose | at pH 6.0 and 37°C | Leuconostoc mesenteroides | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | addition of 0.1 mM Ca2+ in the reaction medium, containing 0.05 mM EDTA, causes a significant increase in the enzyme's activity, achieving about 100% relative activity | Leuconostoc mesenteroides | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Leuconostoc mesenteroides | A0A481MY41 | - |
- |
| Leuconostoc mesenteroides MTCC10508 | A0A481MY41 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| TALON metal affinity resin column chromatography | Leuconostoc mesenteroides |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| jaggary | - |
Leuconostoc mesenteroides | D-glucose + fructose + kestose + nystose + difructose + levan | - |
? | |
| jaggary | - |
Leuconostoc mesenteroides MTCC10508 | D-glucose + fructose + kestose + nystose + difructose + levan | - |
? | |
| sucrose + arabinose | - |
Leuconostoc mesenteroides | ? | - |
? | |
| sucrose + D-xylose | - |
Leuconostoc mesenteroides | ? | - |
? | |
| sucrose + D-xylose | - |
Leuconostoc mesenteroides MTCC10508 | ? | - |
? | |
| sucrose + fructose | - |
Leuconostoc mesenteroides | ? | - |
? | |
| sucrose + glucose | - |
Leuconostoc mesenteroides | ? | - |
? | |
| sucrose + lactose | - |
Leuconostoc mesenteroides | ? | - |
? | |
| sucrose + lactose | - |
Leuconostoc mesenteroides MTCC10508 | ? | - |
? | |
| sucrose + maltose | - |
Leuconostoc mesenteroides | ? | - |
? | |
| sucrose + maltose | - |
Leuconostoc mesenteroides MTCC10508 | ? | - |
? | |
| sucrose + mannose | - |
Leuconostoc mesenteroides | ? | - |
? | |
| sucrose + polygalacturonic acid | - |
Leuconostoc mesenteroides | ? | - |
? | |
| sucrose + raffinose | - |
Leuconostoc mesenteroides | melibiose + D-fructose + levan | - |
? | |
| sucrose + sorbitol | - |
Leuconostoc mesenteroides | ? | - |
? | |
| sucrose + sorbitol | - |
Leuconostoc mesenteroides MTCC10508 | ? | - |
? | |
| sucrose + stachyose | - |
Leuconostoc mesenteroides | manninotriose + D-fructose | - |
? | |
| sucrose + sucrose | - |
Leuconostoc mesenteroides | D-glucose + levan + fructooligosaccharides | - |
? | |
| surgarcane molasse | - |
Leuconostoc mesenteroides | D-glucose + fructose + kestose + nystose + difructose + levan | - |
? | |
| sweet sorghum juice | - |
Leuconostoc mesenteroides | D-glucose + fructose + kestose + nystose + difructose + levan | - |
? | |
| table sugar | - |
Leuconostoc mesenteroides | D-glucose + fructose + kestose + nystose + difructose + levan | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 108000, truncated enzyme, SDS-PAGE | Leuconostoc mesenteroides |
| ? | x * 112076, calculated from amino acid sequence | Leuconostoc mesenteroides |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| LEVS | - |
Leuconostoc mesenteroides |
| sucrose: 2, 6-beta-D-fructan 6-beta-Dfructosyltransferase | - |
Leuconostoc mesenteroides |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
- |
Leuconostoc mesenteroides |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 50 | a gradual loss in the enzyme's activity is recorded with the increase of temperature from 30 to 90°C. The enzyme exhibits 60% of its activity at 50°C, whereas, the activity is completely lost at temperature 70°C and above | Leuconostoc mesenteroides |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | 60 | the enzyme loses about 50% of its activity after incubation for 30 min at 40°C, while the enzyme incubated at 50°C retains about 20% activity. The complete loss in catalytic activity is observed upon incubation at 60°C and higher temperatures | Leuconostoc mesenteroides |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | - |
- |
Leuconostoc mesenteroides |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 6 | the maximum enzymatic activity of the enzyme is obtained in the pH range of 5.0 to 6.0. The enzyme shows 60% activity at pH 4.0, while a significant loss in the enzyme's activity is observed towards the basic pH | Leuconostoc mesenteroides |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Leuconostoc mesenteroides | calculated from amino acid sequence | - |
4.7 |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
