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Literature summary for 2.4.1.10 extracted from

  • Gao, S.; Qi, X.; Hart, D.J.; Gao, H.; An, Y.
    Expression and characterization of levansucrase from Clostridium acetobutylicum (2017), J. Agric. Food Chem., 65, 867-871 .
    View publication on PubMed
Search for more literature for 2.4.1.10

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Clostridium acetobutylicum

Inhibitors

Inhibitors Comment Organism Structure
Ag+ 2% residual activity at 5 mM Clostridium acetobutylicum
Al3+ 55% residual activity at 5 mM Clostridium acetobutylicum
Cu2+ 5% residual activity at 5 mM Clostridium acetobutylicum
EDTA 36% residual activity at 5 mM Clostridium acetobutylicum
Fe2+ 50% residual activity at 5 mM Clostridium acetobutylicum
Hg2+ 2% residual activity at 5 mM Clostridium acetobutylicum
additional information not inhibited by urea Clostridium acetobutylicum
SDS 3% residual activity at 5 mM Clostridium acetobutylicum
Zn2+ 41% residual activity at 5 mM Clostridium acetobutylicum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
64
-
 sucrose at pH 5.9 and 50°C Clostridium acetobutylicum

Metals/Ions

Metals/Ions Comment Organism Structure
Ba2+ 107% activity at 5 mM Clostridium acetobutylicum
Ca2+ 113% activity at 5 mM Clostridium acetobutylicum
K+ 118% activity at 5 mM Clostridium acetobutylicum
Mg2+ 115% activity at 5 mM Clostridium acetobutylicum
Mn2+ 128% activity at 5 mM Clostridium acetobutylicum
additional information not influenced by Rb+, Na+, Co2+ and Li+ Clostridium acetobutylicum
Ni2+ 109% activity at 5 mM Clostridium acetobutylicum
Sn2+ 143% activity at 5 mM Clostridium acetobutylicum

Organism

Organism UniProt Comment Textmining
Clostridium acetobutylicum
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA agarose column chromatography Clostridium acetobutylicum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
sucrose + sucrose the enzyme has high product specificity, and no fructooligosaccharide is identified in the product Clostridium acetobutylicum D-glucose + levan
-
 ?

Synonyms

Synonyms Comment Organism
SacB
-
 Clostridium acetobutylicum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
-
 Clostridium acetobutylicum

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 70 the enzyme activity is greatly reduced at temperatures below 30°C or above 70°C. At temperatures higher than 70°C, the enzyme is inactive Clostridium acetobutylicum

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
20 70 after 30 min incubation, the enzyme retains about 20, 45, 75, 100, 30 and 20% activity at 20, 30, 40, 50, 60 and 70°C, respectively Clostridium acetobutylicum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
 Clostridium acetobutylicum

pH Range

pH Minimum pH Maximum Comment Organism
4 7 the enzyme activity is greatly reduced at pH below 4.0 or above pH 7.0 Clostridium acetobutylicum

pH Stability

pH Stability pH Stability Maximum Comment Organism
4 8 after 30 min incubation, the enzyme retains about 60, 100, 98, 58, and 62% activity at pH 4.0, 5.0, 6.0, 7.0 and 8.0, respectively Clostridium acetobutylicum

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
5
-
 at pH 5.9 and 50°C Clostridium acetobutylicum Fe2+