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Literature summary for 2.4.1.104 extracted from

  • Taguchi, G.; Imura, H.; Maeda, Y.; Kodaira, R.; Hayashida, N.; Shimosaka, M.; Okazaki, M.
    Purification and characterization of UDP-glucose:hydroxycoumarin 7-O-glucosyltransferase, with broad substrate specificity from tobacco cultured cells (2000), Plant Sci., 157, 105-112.
    View publication on PubMed
Search for more literature for 2.4.1.104

Activating Compound

Activating Compound Comment Organism Structure
2-mercaptoethanol 10 mM, activation to 141% of control Nicotiana tabacum
Hg2+
-
 Nicotiana tabacum
PCMB
-
 Nicotiana tabacum

Inhibitors

Inhibitors Comment Organism Structure
Co2+ 1 mM, 87% inhibition Nicotiana tabacum
Cu2+ 1 mM, 55% inhibition Nicotiana tabacum
Zn2+ 1 mM, 96% inhibition Nicotiana tabacum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.025
-
 esculetin
-
 Nicotiana tabacum
0.043
-
 UDPglucose
-
 Nicotiana tabacum
0.15
-
 scopoletin
-
 Nicotiana tabacum

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ 1 mM, activation to 114% of control Nicotiana tabacum
Cu2+ 1 mM, activation to 112% of control Nicotiana tabacum
Mn2+ 1 mM, activation to 133% of control Nicotiana tabacum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
47000 50000 gel filtration Nicotiana tabacum
49000
-
 1 * 49000, SDS-PAGE Nicotiana tabacum

Organism

Organism UniProt Comment Textmining
Nicotiana tabacum
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Nicotiana tabacum

Source Tissue

Source Tissue Comment Organism Textmining
cell suspension culture
-
 Nicotiana tabacum
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
UDP-alpha-D-glucose + kaempferol
-
 Nicotiana tabacum UDP + kaempferol 7-O-beta-D-glucoside
-
 ?
UDP-alpha-D-glucose + quercetin
-
 Nicotiana tabacum UDP + quercetin 7-O-beta-D-glucoside
-
 ?
UDP-glucose + 4-coumaric acid
-
 Nicotiana tabacum UDP + 4-O-glucosylcoumaric acid
-
 ?
UDP-glucose + 4-methylumbelliferone
-
 Nicotiana tabacum UDP + 7-O-glucosyl-4-methylumbelliferone
-
 ?
UDP-glucose + baicalein
-
 Nicotiana tabacum UDP + 7-O-glucosylbaicalein
-
 ?
UDP-glucose + caffeic acid
-
 Nicotiana tabacum UDP + ?
-
 ?
UDP-glucose + esculetin sequential mechanism Nicotiana tabacum UDP + cichoriin
-
 ?
UDP-glucose + ferulic acid low activity Nicotiana tabacum UDP + 4-O-glucosylferulic acid
-
 ?
UDP-glucose + formononetin
-
 Nicotiana tabacum UDP + 7-O-glucosylformononetin
-
 ?
UDP-glucose + scopoletin sequential mechanism Nicotiana tabacum UDP + scopolin
-
 ?
UDP-glucose + umbelliferone
-
 Nicotiana tabacum UDP + skimmin
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 49000, SDS-PAGE Nicotiana tabacum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
-
 Nicotiana tabacum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
 Nicotiana tabacum