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Literature summary for 2.4.1.11 extracted from
- Purification and properties of glycogen synthase I from human leukocytes (1977), Eur. J. Biochem., 81, 119-128.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| glucose 6-phosphate | synthase D, i.e. phosphorylated form, is glucose 6-phosphate dependent, synthase I, i.e. dephosphorylated form, is glucose 6-phosphate independent, interconversion of D and I forms and vice versa | Homo sapiens |  |
General Stability
| General Stability | Organism |
|---|---|
| albumin is essential during lyophilization, lyophilized enzyme is stable for years | Homo sapiens |
| dithiothreitol stabilizes | Homo sapiens |
| glucose 6-phosphate stabilizes | Homo sapiens |
| glycogen stabilizes | Homo sapiens |
| phosphate stabilizes | Homo sapiens |
| spontaneous, temperature-dependent inactivation caused by irreversible conformational changes | Homo sapiens |
| sulfate stabilizes | Homo sapiens |
| UDP stabilizes | Homo sapiens |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2-mercaptoethanol | high concentrations | Homo sapiens | |
| ATP | - |
Homo sapiens | |
| dithiothreitol | high concentrations | Homo sapiens | |
| ethanol | traces inactivate the enzyme | Homo sapiens | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 390000 | - |
sucrose density gradient centrifugation | Homo sapiens |
| 410000 | - |
gel filtration | Homo sapiens |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| UDP-glucose + glycogen | Homo sapiens | - |
glycogen + UDP | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| leukocyte | - |
Homo sapiens | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 21 | - |
synthase I | Homo sapiens |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, glycogen-free or glycogen-containing enzyme, lyophilized, more than 3 years, solubilized enzyme several weeks | Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| UDP-glucose + glycogen | - |
Homo sapiens | glycogen + UDP | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 80000-85000, SDS-PAGE | Homo sapiens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
- |
Homo sapiens |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
inactivation in a few h | Homo sapiens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.8 | 9.2 | synthase I, presence of glucose 6-phosphate | Homo sapiens |
| 7.2 | 8 | synthase I, absence of glucose 6-phosphate | Homo sapiens |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 6.8 | 7.6 | - |
Homo sapiens |
| 7.4 | 8.4 | sensitive to small changes in pH in the presence of Mg2+ | Homo sapiens |
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