Inhibitors | Comment | Organism | Structure |
---|---|---|---|
dithiobis-(2-nitrobenzoic acid) | 77% inactivation at 1 mM | Saccharomyces cerevisiae | |
iodoacetate | 10% inactivation at 10 mM | Saccharomyces cerevisiae | |
iodobenzoate | 22% inactivation at 1 mM | Saccharomyces cerevisiae | |
N-ethylmaleimide | 83% inactivation at 3 mM | Saccharomyces cerevisiae | |
p-chloromercuribenzoate | 90% inactivation at 0.01 mM, dithiothreitol reverses | Saccharomyces cerevisiae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.4 | 1.1 | UDP-glucose | value dependent on glucose 6-phosphate | Saccharomyces cerevisiae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
77000 | - |
4 * 77000, SDS-PAGE | Saccharomyces cerevisiae |
300000 | 310000 | sedimentation equilibrium | Saccharomyces cerevisiae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-glucose + glycogen | Saccharomyces cerevisiae | - |
glycogen + UDP | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
phosphoprotein | incorporation of 1.4 phosphate groups per subunit | Saccharomyces cerevisiae |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Saccharomyces cerevisiae | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
90.5 | - |
- |
Saccharomyces cerevisiae |
Storage Stability | Organism |
---|---|
-20°C, 45 mM Tris/HCl buffer, pH 7.5, 1 mM 2-mercaptoethanol, 10% glycerol, several months | Saccharomyces cerevisiae |
-20°C, stable for several months | Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-glucose + glycogen | - |
Saccharomyces cerevisiae | glycogen + UDP | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 77000, SDS-PAGE | Saccharomyces cerevisiae |