Cloned (Comment) | Organism |
---|---|
the full-length open reading frame of FaGT1 is cloned into the expression vector pET-29a(1) for heterologous protein expression in Escherichia coli | Fragaria x ananassa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Fragaria x ananassa | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant | Fragaria x ananassa |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
fruit | transcripts of FaGT1 are almost undetectable in green fruits. Gene expression increases dramatically in both turning and ripe red fruit, corresponding closely to the accumulation of anthocyanins during fruit ripening. The expression of FaGT1 is fruit associated and negatively regulated by auxin | Fragaria x ananassa | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | no activity with: 3-hydroxyflavone, morin, catechin, epicatechin, naringenin, taxifolin, 5-hydroxyflavone, 7-hydroxyflavone, chrysin, apigenin, quercetin, kaempferol 3-O-glucoside, and anthocyanins. FaGT1 acts exclusively on anthocyanidins and flavonols in vitro. No product is formed when UDP-Gal and UDP-GlcUA are used as sugar donors with pelargonidin or quercetin as acceptor molecules | Fragaria x ananassa | ? | - |
? | |
UDP-alpha-D-glucose + 3,7-dihydroxyflavone | about 10% of the activity with cyanidin | Fragaria x ananassa | UDP + 3-[(beta-D-glucopyranosyl)oxy]-7-hydroxyflavone | - |
? | |
UDP-alpha-D-glucose + isorhamnetin | about 25% of the activity with cyanidin | Fragaria x ananassa | UDP + isorhamnetin 3-O-beta-D-glucoside | - |
? | |
UDP-glucose + delphinidin | about 70% of the activity with cyanidin | Fragaria x ananassa | UDP + delphinidin 3-O-glucoside | - |
? | |
UDP-glucose + pelargonidin | about 90% of the activity with cyanidin | Fragaria x ananassa | UDP + pelargonidin 3-O-glucoside | - |
? | |
UDPalpha-D-glucose + galangin | about 15% of the activity with cyanidin | Fragaria x ananassa | UDP + galangin 3-O-bbeta-D-glucoside | - |
? | |
UDPglucose + cyanidin | - |
Fragaria x ananassa | UDP + cyanidin 3-O-beta-D-glucoside | - |
? | |
UDPglucose + fisetin | about 10% of the activity with cyanidin | Fragaria x ananassa | UDP + fisetin 3-O-glucoside | - |
? | |
UDPglucose + kaempferol | about 5% of the activity with cyanidin | Fragaria x ananassa | UDP + kaempferol 3-O-glucoside | - |
? | |
UDPglucose + malvidin | about 20% of the activity with cyanidin | Fragaria x ananassa | UDP + malvidin 3-O-glucoside | - |
? | |
UDPglucose + myricetin | about 15% of the activity with cyanidin | Fragaria x ananassa | UDP + 3-O-glucoside myricetin | - |
? | |
UDPglucose + peonidin | about 85% of the activity with cyanidin | Fragaria x ananassa | UDP + peonidin 3-O-beta-D-glucoside | - |
? | |
UDPglucose + petunidin | about 35% of the activity with cyanidin | Fragaria x ananassa | UDP + petunidin 3-O-glucoside | - |
? | |
UDPglucose + quercetin | about 15% of the activity with cyanidin | Fragaria x ananassa | UDP + quercetin 3-O-glucoside | - |
? |
Synonyms | Comment | Organism |
---|---|---|
anthocyanidin-3-O-glucosyltransferase | - |
Fragaria x ananassa |
FaGT1 | - |
Fragaria x ananassa |