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Literature summary for 2.4.1.115 extracted from

  • Griesser, M.; Hoffmann, T.; Bellido, M.L.; Rosati, C.; Fink, B.; Kurtzer, R.; Aharoni, A.; Munoz-Blanco, J.; Schwab, W.
    Redirection of flavonoid biosynthesis through the down-regulation of an anthocyanidin glucosyltransferase in ripening strawberry fruit (2008), Plant Physiol., 146, 1528-1539.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
the full-length open reading frame of FaGT1 is cloned into the expression vector pET-29a(1) for heterologous protein expression in Escherichia coli Fragaria x ananassa

Organism

Organism UniProt Comment Textmining
Fragaria x ananassa
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-
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Purification (Commentary)

Purification (Comment) Organism
recombinant Fragaria x ananassa

Source Tissue

Source Tissue Comment Organism Textmining
fruit transcripts of FaGT1 are almost undetectable in green fruits. Gene expression increases dramatically in both turning and ripe red fruit, corresponding closely to the accumulation of anthocyanins during fruit ripening. The expression of FaGT1 is fruit associated and negatively regulated by auxin Fragaria x ananassa
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information no activity with: 3-hydroxyflavone, morin, catechin, epicatechin, naringenin, taxifolin, 5-hydroxyflavone, 7-hydroxyflavone, chrysin, apigenin, quercetin, kaempferol 3-O-glucoside, and anthocyanins. FaGT1 acts exclusively on anthocyanidins and flavonols in vitro. No product is formed when UDP-Gal and UDP-GlcUA are used as sugar donors with pelargonidin or quercetin as acceptor molecules Fragaria x ananassa ?
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?
UDP-alpha-D-glucose + 3,7-dihydroxyflavone about 10% of the activity with cyanidin Fragaria x ananassa UDP + 3-[(beta-D-glucopyranosyl)oxy]-7-hydroxyflavone
-
?
UDP-alpha-D-glucose + isorhamnetin about 25% of the activity with cyanidin Fragaria x ananassa UDP + isorhamnetin 3-O-beta-D-glucoside
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?
UDP-glucose + delphinidin about 70% of the activity with cyanidin Fragaria x ananassa UDP + delphinidin 3-O-glucoside
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?
UDP-glucose + pelargonidin about 90% of the activity with cyanidin Fragaria x ananassa UDP + pelargonidin 3-O-glucoside
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?
UDPalpha-D-glucose + galangin about 15% of the activity with cyanidin Fragaria x ananassa UDP + galangin 3-O-bbeta-D-glucoside
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?
UDPglucose + cyanidin
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Fragaria x ananassa UDP + cyanidin 3-O-beta-D-glucoside
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?
UDPglucose + fisetin about 10% of the activity with cyanidin Fragaria x ananassa UDP + fisetin 3-O-glucoside
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?
UDPglucose + kaempferol about 5% of the activity with cyanidin Fragaria x ananassa UDP + kaempferol 3-O-glucoside
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?
UDPglucose + malvidin about 20% of the activity with cyanidin Fragaria x ananassa UDP + malvidin 3-O-glucoside
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?
UDPglucose + myricetin about 15% of the activity with cyanidin Fragaria x ananassa UDP + 3-O-glucoside myricetin
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?
UDPglucose + peonidin about 85% of the activity with cyanidin Fragaria x ananassa UDP + peonidin 3-O-beta-D-glucoside
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?
UDPglucose + petunidin about 35% of the activity with cyanidin Fragaria x ananassa UDP + petunidin 3-O-glucoside
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?
UDPglucose + quercetin about 15% of the activity with cyanidin Fragaria x ananassa UDP + quercetin 3-O-glucoside
-
?

Synonyms

Synonyms Comment Organism
anthocyanidin-3-O-glucosyltransferase
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Fragaria x ananassa
FaGT1
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Fragaria x ananassa