Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Reducing agent | required | Acanthamoeba castellanii |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EDTA | inhibition reversed by MgCl2 | Acanthamoeba castellanii | |
UDP | competitive | Acanthamoeba castellanii | |
UMP | reversible | Acanthamoeba castellanii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0045 | - |
dolichyl phosphate | - |
Acanthamoeba castellanii | |
0.0091 | - |
UDP-glucose | - |
Acanthamoeba castellanii |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
soluble | - |
Acanthamoeba castellanii | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | divalent cation required, either Mg2+ or Mn2+, Mn2+ stimulates the enzyme to a greater extent than Mg2+ at low concentrations, Mg2+ stimulates to a greater extent than Mn2+ at high concentration, but each is maximally effective at a concentration around 8 mM | Acanthamoeba castellanii | |
Mn2+ | divalent cation required, either Mg2+ or Mn2+, Mn2+ stimulates the enzyme to a greater extent than Mg2+ at low concentrations, Mg2+ stimulates to a greater extent than Mn2+ at high concentration, but each is maximally effective at a concentration around 8 mM | Acanthamoeba castellanii |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Acanthamoeba castellanii | synthesis of dolichyl D-glucosyl phosphate, a glucosyl donor in the formation of lipid-linked core oligosaccharides | ? | - |
? | |
UDP-glucose + dolichyl phosphate | Acanthamoeba castellanii | - |
UDP + dolichyl beta-D-glucosyl phosphate | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Acanthamoeba castellanii | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cyst | - |
Acanthamoeba castellanii | - |
Storage Stability | Organism |
---|---|
-20°C, indefinitely stable below | Acanthamoeba castellanii |
4°C, stable for several days | Acanthamoeba castellanii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | no substrate: GDP-D-glucose, UDP-D-glucuronic acid, UDP-N-acetyl-D-glucosamine, UDP-D-xylose | Acanthamoeba castellanii | ? | - |
? | |
additional information | synthesis of dolichyl D-glucosyl phosphate, a glucosyl donor in the formation of lipid-linked core oligosaccharides | Acanthamoeba castellanii | ? | - |
? | |
UDP-glucose + dolichyl phosphate | - |
Acanthamoeba castellanii | UDP + dolichyl beta-D-glucosyl phosphate | - |
r | |
UDP-glucose + ficaprenyl phosphate | - |
Acanthamoeba castellanii | UDP + ficaprenyl beta-D-glucosyl phosphate | - |
r | |
UDP-glucose + solanesyl phosphate | - |
Acanthamoeba castellanii | UDP + solanesyl beta-D-glucosyl phosphate | - |
r |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
- |
Acanthamoeba castellanii |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
2 h, 50% loss of activity | Acanthamoeba castellanii |
52 | - |
10 min, complete loss of activity | Acanthamoeba castellanii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Acanthamoeba castellanii |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.6 | 8.8 | pH 5.6: 25% of maximum activity, pH 8.8: about 40% of maximum activity | Acanthamoeba castellanii |