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Literature summary for 2.4.1.120 extracted from
- Cloning and heterologous expression of a rape cDNA encoding UDP-glucose:sinapate glucosyltransferase (2000), Planta, 211, 883-886.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| construction of a cDNA library, DNA and amino acid sequence determination and analysis, functional expression in Escherichia coli strain M15 as soluble protein | Brassica napus |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 55970 | - |
x * 55970, amino acid sequence calculation, mass spectrometry | Brassica napus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| UDP-alpha-D-glucose + sinapate | Brassica napus | - |
UDP + 1-O-sinapoyl-beta-D-glucose | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Brassica napus | Q9FYU7 | SGT1 | - |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| cotyledon | from 3-days-old seedlings | Brassica napus | - |
| seedling | - |
Brassica napus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.018 | - |
recombinant enzyme, substrate sinapate | Brassica napus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | recombinant enzyme shows a broad substrate specificity, indole-3-acetate, 4-hydroxybenzoate, and salicylate are no substrates | Brassica napus | ? | - |
? | |
| UDP-alpha-D-glucose + 4-coumarate | 13% activity compared to sinapate | Brassica napus | UDP + 1-O-(4-coumaroyl)-beta-D-glucose | - |
? | |
| UDP-alpha-D-glucose + cinnamate | 20% activity compared to sinapate | Brassica napus | UDP + 1-O-cinnamoyl-beta-D-glucose | - |
? | |
| UDP-alpha-D-glucose + ferulate | 17% activity compared to sinapate | Brassica napus | UDP + 1-O-feruloyl-beta-D-glucose | - |
? | |
| UDP-alpha-D-glucose + sinapate | - |
Brassica napus | UDP + 1-O-sinapoyl-beta-D-glucose | - |
? | |
| UDP-alpha-D-glucose + sinapate | best substrate | Brassica napus | UDP + 1-O-sinapoyl-beta-D-glucose | - |
? | |
| UDP-glucose + caffeate | 10% activity compared to sinapate | Brassica napus | UDP + 1-caffeoyl-D-glucose | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 55970, amino acid sequence calculation, mass spectrometry | Brassica napus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| SGT | - |
Brassica napus |
| UDP-glucose:sinapate glucosyltransferase | - |
Brassica napus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Brassica napus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
assay at | Brassica napus |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Brassica napus | sequence calculation | - |
6.1 |
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Release 2023.1 (January 2023)
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