Activating Compound | Comment | Organism | Structure |
---|---|---|---|
D-gluconic acid lactone | the inhibitor of beta-glucosidase activity, potentiates the enzyme activity at the optimal concentration of 0.3 mM | Pisum sativum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-naphthalene acetic acid | competitive inhibitor | Pisum sativum | |
indole-3-butyric acid | competitive inhibitor | Pisum sativum | |
Indole-3-propionic acid | competitive inhibitor | Pisum sativum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.52 | - |
(indol-3-yl)acetate | pH 7.6, 30°C | Pisum sativum | |
1.5 | - |
(indol-3-yl)propionate | pH 7.6, 30°C | Pisum sativum | |
2.67 | - |
UDP-glucose | pH 7.6, 30°C | Pisum sativum | |
4 | - |
1-naphthalene acetate | pH 7.6, 30°C | Pisum sativum |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Pisum sativum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-glucose + (indol-3-yl)acetate | Pisum sativum | - |
UDP + 1-O-(indol-3-yl)acetyl-beta-D-glucose | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pisum sativum | - |
- |
- |
Purification (Comment) | Organism |
---|---|
native enzyme from immature seeds by PEG fractionation, anion exchange chromatography, and preparative PAGE | Pisum sativum |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
seed | immature | Pisum sativum | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.06 | - |
purified enzyme, substrate gibberellin GA3, pH 7.6, 30°C | Pisum sativum |
0.656 | - |
purified enzyme, substrate (indol-3-yl)propionate, pH 7.6, 30°C | Pisum sativum |
0.715 | - |
purified enzyme, substrate 1-naphthalene acetate, pH 7.6, 30°C | Pisum sativum |
2.976 | - |
purified enzyme, substrate (indol-3-yl)acetate, pH 7.6, 30°C | Pisum sativum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-glucose + (indol-3-yl)acetate | - |
Pisum sativum | UDP + 1-O-(indol-3-yl)acetyl-beta-D-glucose | - |
r | |
UDP-glucose + (indol-3-yl)acetate | best substrate | Pisum sativum | UDP + 1-O-(indol-3-yl)acetyl-beta-D-glucose | - |
r | |
UDP-glucose + (indol-3-yl)propionate | 22% of the activity with (indol-3-yl)acetate | Pisum sativum | UDP + 1-O-(indol-3-yl)propionyl-beta-D-glucose | - |
r | |
UDP-glucose + 1-naphthalene acetate | 24% of the activity with (indol-3-yl)acetate | Pisum sativum | UDP + 1-naphthalene acetyl-beta-D-glucose | - |
r | |
UDP-glucose + gibberellin GA3 | 1.9% of the activity with (indol-3-yl)acetate | Pisum sativum | UDP + ? | - |
r |
Synonyms | Comment | Organism |
---|---|---|
IAA glucosyltransferase | - |
Pisum sativum |
IAGlc synthase | - |
Pisum sativum |
indole-3-acetic acid UDP-glucosyltransferase | - |
Pisum sativum |
UDPG: indole-3-ylacetyl-beta-D-glucosyltransferase | - |
Pisum sativum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Pisum sativum |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.49 | - |
UDP-glucose | pH 7.6, 30°C | Pisum sativum | |
0.53 | - |
1-naphthalene acetate | pH 7.6, 30°C | Pisum sativum | |
0.72 | - |
(indol-3-yl)propionate | pH 7.6, 30°C | Pisum sativum | |
2.27 | - |
(indol-3-yl)acetate | pH 7.6, 30°C | Pisum sativum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.6 | - |
- |
Pisum sativum |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.038 | - |
2-naphthalene acetic acid | pH 7.6, 30°C | Pisum sativum | |
0.1 | - |
indole-3-butyric acid | pH 7.6, 30°C | Pisum sativum | |
0.15 | - |
Indole-3-propionic acid | pH 7.6, 30°C | Pisum sativum |
General Information | Comment | Organism |
---|---|---|
evolution | the enzyme belongs to the UDP-sugar dependent transferases | Pisum sativum |
metabolism | the enzyme catalyzes the first step in the biosynthesis of indol-3-yl ester conjugates in monocotyledonous plants | Pisum sativum |
physiological function | 1-O-(indol-3-yl)acetyl-beta-D-glucose, synthesized by the enzyme, is the substrate for (indol-3-yl)acetate labeling of glycoproteins from pea seeds indicating a possible role of this enzyme in the covalent modification of a class of proteins by a plant hormone. Auxin is a key plant hormone that regulates many physiological processes in plants including organ morphogenesis (i.e. apical dominance, root development) and environmental responses (biotic and abiotic stress). The major auxin is indole-3-acetic acid (IAA), but other indole-derived carboxylic acids, i.e. indole-3-butyric acid, indole-3-propionic acid, 4-chloro-indole-3-acetic acid, and indole-3-pyruvic acid have also auxin activity. Auxin action is highly dependent on its concentration, which must be precisely controlled | Pisum sativum |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.13 | - |
1-naphthalene acetate | pH 7.6, 30°C | Pisum sativum | |
0.16 | - |
UDP-glucose | pH 7.6, 30°C | Pisum sativum | |
0.46 | - |
(indol-3-yl)propionate | pH 7.6, 30°C | Pisum sativum | |
4.36 | - |
(indol-3-yl)acetate | pH 7.6, 30°C | Pisum sativum |