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Literature summary for 2.4.1.128 extracted from
- A novel glucosyltransferase from Catharanthus roseus cell suspensions (2010), Process Biochem., 45, 655-659.
No PubMed abstract available
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Cu2+ | 51.5% residual activity at 1 mM, 12% residual activity at 10 mM | Catharanthus roseus |  |
| Mn2+ | 40.9% residual activity at 1 mM, complete inhibition at 10 mM | Catharanthus roseus | |
| Zn2+ | 27.1% residual activity at 1 mM, complete inhibition at 10 mM | Catharanthus roseus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.112 | - |
scopoletin | at 37°C, pH not specified in the publication | Catharanthus roseus | |
| 1 | - |
UDP-glucose | at 37°C, pH not specified in the publication | Catharanthus roseus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| additional information | not affected by Mg2+ and Ca2+ | Catharanthus roseus | |
| Na+ | the addition of Na+ to buffered standard assays at concentration 1 and 10 mM has a stimulating effect on the glucosylation of scopoletin to 110% and 128%, respectively | Catharanthus roseus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 51000 | - |
x * 51000, SDS-PAGE | Catharanthus roseus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Catharanthus roseus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| ammonium sulfate precipitation, phenyl-Sepharose column chromatographyand DEAE Sepharose column chromatography | Catharanthus roseus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| callus | - |
Catharanthus roseus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | the enzyme shows activity towards a number of coumarins including umbelliferone, scopoletin, isoscopoletin and esculetin, and flavonoids including a flavone, a flavanone and chalcones. No activity is detected with compounds characterized by a single aromatic ring, i.e. simple and acidic phenols | Catharanthus roseus | ? | - |
? | |
| UDP-glucose + scopoletin | - |
Catharanthus roseus | UDP + scopolin | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 51000, SDS-PAGE | Catharanthus roseus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 35 | - |
- |
Catharanthus roseus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
in Tris buffer | Catharanthus roseus |
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Release 2023.1 (January 2023)
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