Activating Compound | Comment | Organism | Structure |
---|---|---|---|
dolichol | stimulates enzyme in the presence of inhibitory concentrations of phosphatidylcholine | Oryctolagus cuniculus | |
dolichyl phosphate | stimulates enzyme in the presence of inhibitory concentrations of phosphatidylcholine | Oryctolagus cuniculus | |
Nonidet P-40 | activation, up to 0.0225% v/v | Oryctolagus cuniculus |
General Stability | Organism |
---|---|
glycerol, 0.5% v/v, stabilizes during storage and purification | Oryctolagus cuniculus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ADP | weak | Oryctolagus cuniculus | |
ATP | weak | Oryctolagus cuniculus | |
EDTA | - |
Oryctolagus cuniculus | |
GDP | 50% inhibition at 0.0006 mM | Oryctolagus cuniculus | |
GDPglucose | - |
Oryctolagus cuniculus | |
glycerol | 1-2% v/v | Oryctolagus cuniculus | |
GMP | 50% inhibition at 0.094 mM | Oryctolagus cuniculus | |
GTP | 50% inhibition at 0.006 mM | Oryctolagus cuniculus | |
additional information | no inhibition by amphomycin | Oryctolagus cuniculus | |
NiCl2 | - |
Oryctolagus cuniculus | |
UDP | weak | Oryctolagus cuniculus | |
UDP-N-acetylglucosamine | weak | Oryctolagus cuniculus | |
UDPglucose | weak | Oryctolagus cuniculus | |
UTP | weak | Oryctolagus cuniculus | |
ZnCl2 | - |
Oryctolagus cuniculus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00021 | - |
tetrasaccharide-diphosphoryl-lipid | detergent-stimulated | Oryctolagus cuniculus | |
0.00065 | - |
GDPmannose | detergent-stimulated | Oryctolagus cuniculus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
microsome | - |
Oryctolagus cuniculus | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | activation, about 60% as effective as Mg2+ | Oryctolagus cuniculus | |
Co2+ | activation, about 4% as effective as Mg2+ | Oryctolagus cuniculus | |
Mg2+ | requirement | Oryctolagus cuniculus | |
Mg2+ | 10 mM | Oryctolagus cuniculus | |
Mn2+ | activation, about 40% as effective as Mg2+ | Oryctolagus cuniculus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
GDPmannose + tetrasaccharide-diphosphoryl-lipid | Oryctolagus cuniculus | involved in the biosynthesis of asparagine-linked saccharide chains of mammalian glycoproteins | GDP + mannosyl-alpha-1,3-tetrasaccharide-diphosphoryl-lipid | - |
? | |
GDPmannose + tetrasaccharide-diphosphoryl-lipid | Oryctolagus cuniculus | the 1,3-linked mannosyl residue in mammalian lipid-linked oligosaccharide of the structure Glc3Man9GlcNAc2 is produced by this enzyme | GDP + mannosyl-alpha-1,3-tetrasaccharide-diphosphoryl-lipid | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Oryctolagus cuniculus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
partial | Oryctolagus cuniculus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Oryctolagus cuniculus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Oryctolagus cuniculus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
GDPmannose + tetrasaccharide-diphosphoryl-lipid | no acceptor is dolichol-phosphate | Oryctolagus cuniculus | GDP + mannosyl-alpha-1,3-tetrasaccharide-diphosphoryl-lipid | - |
? | |
GDPmannose + tetrasaccharide-diphosphoryl-lipid | involved in the biosynthesis of asparagine-linked saccharide chains of mammalian glycoproteins | Oryctolagus cuniculus | GDP + mannosyl-alpha-1,3-tetrasaccharide-diphosphoryl-lipid | - |
? | |
GDPmannose + tetrasaccharide-diphosphoryl-lipid | the 1,3-linked mannosyl residue in mammalian lipid-linked oligosaccharide of the structure Glc3Man9GlcNAc2 is produced by this enzyme | Oryctolagus cuniculus | GDP + mannosyl-alpha-1,3-tetrasaccharide-diphosphoryl-lipid | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Oryctolagus cuniculus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.8 | 7.3 | - |
Oryctolagus cuniculus |