Any feedback?
Literature summary for 2.4.1.14 extracted from
- Sucrose phosphate synthetase, separation from sucrose synthetase and a study of its properties (1978), Planta, 142, 41-48.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | stimulates enzyme activity | Triticum aestivum |  |
| Mn2+ | stimulates enzyme activity | Triticum aestivum | |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| ADP | slight inhibition | Triticum aestivum | |
| ATP | - |
Triticum aestivum | |
| citrate | activity can by restored with Mg2+ | Triticum aestivum | |
| CTP | - |
Triticum aestivum | |
| delta-gluconolactone | - |
Triticum aestivum | |
| EDTA | - |
Triticum aestivum | |
| GTP | - |
Triticum aestivum | |
| Maleate | - |
Triticum aestivum | |
| p-chloromercuribenzoate | activity can be restored by addition of DTT or 2-mercaptoethanol | Triticum aestivum | |
| phosphate | activity can by restored with Mg2+ | Triticum aestivum | |
| UDP | competitive with UDP-glucose | Triticum aestivum | |
| UTP | - |
Triticum aestivum | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | reverses inhibition with citrate, phosphate and nucloside triphosphates | Triticum aestivum | |
| Mn2+ | stimulates enzyme activity | Triticum aestivum | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 380000 | 390000 | gel filtration, glycerol density gradient centrifugation | Triticum aestivum |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| UDP-alpha-D-glucose + D-fructose 6-phosphate | Triticum aestivum | catalyzes the penultimate step of sucrose synthesis | UDP + sucrose 6-phosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Triticum aestivum | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| complete separation from sucrose synthetase | Triticum aestivum |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| germ | - |
Triticum aestivum | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.68 | - |
purified enzyme | Triticum aestivum |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, 1 month, no loss of activity, 1 year, 50-60% loss of activity | Triticum aestivum |
| 0-4°C, stable in presence of 20% glycerol or 5 mM fructose-6-phosphate | Triticum aestivum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| UDP-alpha-D-glucose + D-fructose 6-phosphate | - |
Triticum aestivum | UDP + sucrose 6-phosphate | - |
? | |
| UDP-alpha-D-glucose + D-fructose 6-phosphate | catalyzes the penultimate step of sucrose synthesis | Triticum aestivum | UDP + sucrose 6-phosphate | - |
? | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | - |
- |
Triticum aestivum |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
