Cloned (Comment) | Organism |
---|---|
gene SPS1, recombinant expression driven by the CaMV35S promoter in leaves of Nicotiana tabacum via Agrobacterium tumefaciens strain GV3101 transfection method, coexpression with soybean glutamine synthetase | Zea mays |
Protein Variants | Comment | Organism |
---|---|---|
additional information | production of transgenic tobacco plants with a cytosolic soybean glutamine synthetase (GS1) gene and a sucrose phosphate synthase (SPS) gene from maize, both driven by the CaMV35S promoter. The SPS enzyme activity in the SPS and SPS/GS1 co-transformants are the same under both nitrogen regimens, low and high nitrogen. The GS1/SPS transformants show a phenotype similar to the SPS transformants, phenotype overview | Zea mays |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Zea mays |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-glucose + D-fructose 6-phosphate | Zea mays | - |
UDP + sucrose 6F-phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Zea mays | P31927 | gene SPS1 | - |
Purification (Comment) | Organism |
---|---|
recombinant enzyme partially from Nicotiana tabacum leaves | Zea mays |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-glucose + D-fructose 6-phosphate | - |
Zea mays | UDP + sucrose 6F-phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
SPS | - |
Zea mays |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Zea mays |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
95 | - |
purified enzyme, 10 min, inactivation | Zea mays |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Zea mays |
General Information | Comment | Organism |
---|---|---|
metabolism | the enzyme plays a key role in carbon metabolism by catalyzing the synthesis of sucrose | Zea mays |