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Literature summary for 2.4.1.142 extracted from

  • Kaushal, G.P.; Elbein, A.D.
    Partial purification and characterization of beta-mannosyltransferase from suspension-cultured soybean cells (1987), Biochemistry, 26, 7953-7960.
    View publication on PubMed
Search for more literature for 2.4.1.142

Activating Compound

Activating Compound Comment Organism Structure
NP-40
-
 Glycine max
phosphatidic acid
-
 Glycine max
phosphatidylcholine
-
 Glycine max
phosphatidylserine
-
 Glycine max
Phospholipid
-
 Glycine max
Triton X-100 optimum activity at 0.08-0.1% Glycine max

Inhibitors

Inhibitors Comment Organism Structure
GDP
-
 Glycine max
GDP-glucose
-
 Glycine max
GMP
-
 Glycine max
GTP
-
 Glycine max

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0017
-
 GDP-mannose
-
 Glycine max

Localization

Localization Comment Organism GeneOntology No. Textmining
microsome
-
 Glycine max
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ requirement for activity Glycine max

Organism

Organism UniProt Comment Textmining
Glycine max
-
-
-

Purification (Commentary)

Purification (Comment) Organism
DEAE-cellulose, GDP-affinity column Glycine max

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Glycine max

Storage Stability

Storage Stability Organism
frozen, Tris-buffer, pH 7.0, 0.5 mM DTT, 20% glycerol, 0.1% detergent, 1 month Glycine max

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
GDP-alpha-D-mannose + chitobiosyldiphosphodolichol
-
 Glycine max GDP + beta-(1->4)-D-mannosylchitobiosyldiphosphodolichol
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.9 7
-
 Glycine max