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Literature summary for 2.4.1.15 extracted from

  • Lee, J.S.; Hai, T.; Pape, H.; Kim, T.J.; Suh, J.W.
    Three trehalose synthetic pathways in the acarbose-producing Actinoplanes sp. SN223/29 and evidence for the TreY role in biosynthesis of component C (2008), Appl. Microbiol. Biotechnol., 80, 767-778.
    View publication on PubMed
Search for more literature for 2.4.1.15

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Actinoplanes sp.

General Stability

General Stability Organism
in phosphate or acetate buffers, the activity of TpS1 is decreased to 25% and 30%, respectively, of activity in Tris-HCl buffer Actinoplanes sp.

Inhibitors

Inhibitors Comment Organism Structure
EDTA
-
 Actinoplanes sp.
additional information not inhibitory: 2-mercaptoethanol Actinoplanes sp.

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ 95% stimulation Actinoplanes sp.
Mn2+ 20% stimulation Actinoplanes sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
51000
-
 x * 51000, calculated Actinoplanes sp.

Organism

Organism UniProt Comment Textmining
Actinoplanes sp. A8QX04
-
-
Actinoplanes sp. SN223/29 A8QX04
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ADP-alpha-D-glucose + D-glucose 6-phosphate 4% of the activity with UDP-glucose Actinoplanes sp. ADP + alpha,alpha-1,1-trehalose 6-phosphate
-
 ?
ADP-alpha-D-glucose + D-glucose 6-phosphate 4% of the activity with UDP-glucose Actinoplanes sp. SN223/29 ADP + alpha,alpha-1,1-trehalose 6-phosphate
-
 ?
GDP-alpha-D-glucose + D-glucose 6-phosphate 23% of the activity with UDP-glucose Actinoplanes sp. GDP + alpha,alpha-1,1-trehalose 6-phosphate
-
 ?
GDP-alpha-D-glucose + D-glucose 6-phosphate 23% of the activity with UDP-glucose Actinoplanes sp. SN223/29 GDP + alpha,alpha-1,1-trehalose 6-phosphate
-
 ?
additional information no substrate: CDP-glucose, fructose 6-phosphate Actinoplanes sp. ?
-
 ?
additional information no substrate: CDP-glucose, fructose 6-phosphate Actinoplanes sp. SN223/29 ?
-
 ?
UDP-alpha-D-glucose + D-glucose 6-phosphate
-
 Actinoplanes sp. UDP + alpha,alpha-1,1-trehalose 6-phosphate
-
 ?
UDP-alpha-D-glucose + D-glucose 6-phosphate
-
 Actinoplanes sp. SN223/29 UDP + alpha,alpha-1,1-trehalose 6-phosphate
-
 ?

Subunits

Subunits Comment Organism
? x * 51000, calculated Actinoplanes sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
35
-
-
 Actinoplanes sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
 Actinoplanes sp.