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Literature summary for 2.4.1.15 extracted from

  • Petitjean, M.; Teste, M.A.; Francois, J.M.; Parrou, J.L.
    Yeast tolerance to various stresses relies on the trehalose-6P synthase (Tps1) protein, not on trehalose (2015), J. Biol. Chem., 290, 16177-16190.
    View publication on PubMedView publication on EuropePMC

Protein Variants

Protein Variants Comment Organism
D156G site-directed mutagenesis of a catalytic site residue, catalytically inactive Tps1 mutant variant Saccharomyces cerevisiae
additional information construction of a tps1DELTA and a tps1-156 deletion mutant Saccharomyces cerevisiae
W111S site-directed mutagenesis of a catalytic site residue Saccharomyces cerevisiae
Y102V site-directed mutagenesis of a catalytic site residue Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
UDP-glucose + D-glucose 6-phosphate Saccharomyces cerevisiae
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UDP + alpha,alpha-trehalose 6-phosphate
-
?

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae Q00764
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
UDP-glucose + D-glucose 6-phosphate
-
Saccharomyces cerevisiae UDP + alpha,alpha-trehalose 6-phosphate
-
?

Synonyms

Synonyms Comment Organism
TPS1
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Saccharomyces cerevisiae
trehalose-6P synthase
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Saccharomyces cerevisiae

General Information

General Information Comment Organism
malfunction stress hypersensitivity of mutants deleted for gene TPS1. At 42°C, the viability of the tps1DELTA mutant sharply drops by several decades, whereas the viability of the wild-type TPS1 strain remains almost unchanged. The the mal- BY4741 strain is unable to import trehalose. Overview of the impact of the different stresses on the drop of viability in wild-type and mdeletion mutants. The tps1DELTA mutant rapidly loses its ATP content in response to heat shock at 42°C Saccharomyces cerevisiae
metabolism gene TPS1 encodes the first enzyme in trehalose biosynthetic pathway, trehalose is a stress protectant Saccharomyces cerevisiae
physiological function the Tps1 protein is essential to maintain ATP levels during heat shock. Tps1 is endowed with a regulatory function in energy homeostasis, which is essential to withstand adverse conditions and maintain cellular integrity. Tps1, not trehalose, is important for yeast viability in response to various kinds of stresses. Contribution of the Tps1 protein to thermotolerance and acquired thermotolerance, enzyme deletion mutants show highly reduced growth at 42°C in contrast to the wild-type enzyme Saccharomyces cerevisiae