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Literature summary for 2.4.1.152 extracted from

  • Foster, C.S.; Gillies, D.R.B.; Glick, M.C.
    Purification and characterization of GDP-L-Fuc-N-acetyl-beta-D-glucosaminide alpha1,3 fucosyltransferase from human neuroblastoma cells. Unusual substrate specificities of the tumor enzyme (1991), J. Biol. Chem., 266, 3526-3531.
    View publication on PubMed
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Inhibitors

Inhibitors Comment Organism Structure
GMP 80% inhibition at 0.4 mM Homo sapiens
N-ethylmaleimide not inhibitory at 5 mM, 30 min. Homo sapiens
NaCl 40% inhibition at 300 mM Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.043
-
 GDP-fucose
-
 Homo sapiens
0.4
-
 Galbeta1-4GlcNAc
-
 Homo sapiens
1
-
 Fucalpha1-2Galbeta1-4Glc
-
 Homo sapiens
8.1
-
 Galbeta1-4Glc
-
 Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+ optimal activity at 5 mM Homo sapiens
Zn2+ at 10 mM stimulates the activity more than Mn2+ Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
using method A: column chromatography on DEAE-Sephadex, SP-Sephadex, ASTD-Sepharose and GTP-Agarose, method B: column chromatography on DEAE-Sephacel, SP-Sephadex and Synsorb-Galbeta1-4GlcNAc Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
neuroblastoma cell CHP 134 Homo sapiens
-
serum
-
 Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
GDP-fucose + Fucalpha1-2Galbeta1-4GlcNAc
-
 Homo sapiens GDP + Fucalpha1-2Galbeta1-4(Fucalpha1-3)GlcNAc
-
 ?
GDP-fucose + Galbeta1-4Glc
-
 Homo sapiens GDP + Galbeta1-4 (Fucalpha1-3)Glc
-
 ?
GDP-fucose + Galbeta1-4GlcNAc
-
 Homo sapiens GDP + Galbeta1-4 (Fucalpha1-3)GlcNAc
-
 ?
GDP-L-fucose + N4-[N-acetyl-beta-D-glucosaminyl-(1-2)-alpha-D-mannosyl-(1-3)-[N-acetyl-beta-D-glucosaminyl-(1-2)-alpha-D-mannosyl-(1-6)]-beta-D-mannosyl-(1-4)-N-acetyl-beta-D-glucosaminyl-(1-4)-N-acetyl-beta-D-glucosaminyl]asparagine
-
 Homo sapiens GDP + N4-[N-acetyl-beta-D-glucosaminyl-(1-2)-alpha-D-mannosyl-(1-3)-[N-acetyl-beta-D-glucosaminyl-(1-2)-alpha-D-mannosyl-(1-6)]-beta-D-mannosyl-(1-4)-N-acetyl-beta-D-glucosaminyl-(1-4)-[alpha-L-fucosyl-(1-3)]-N-acetyl-beta-D-glucosaminyl]asparagine
-
 ?
additional information
-
 Homo sapiens ?
-
 ?

Subunits

Subunits Comment Organism
? x * 40000-45000, SDS-PAGE Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.25
-
-
 Homo sapiens