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Literature summary for 2.4.1.152 extracted from

  • DeBose-Boyd, R.A.; Nyame, A.K.; Cummings, R.D.
    Molecular cloning and characterization of an alpha1,3 fucosyltransferase, CEFT-1, from Caenorhabditis elegans (1998), Glycobiology, 8, 905-917.
    View publication on PubMed
Search for more literature for 2.4.1.152

Cloned(Commentary)

Cloned (Comment) Organism
putative cDNA amplified by PCR from a cDNAlambdaZAP library, cloned into the mammalian expression vector pCR3.1 and sequenced, transfection of COS7 kidney cells from the African green monkey with cDNA encoding the enzyme results in about 20fold level of activity over control cells, using lacto-N-neotetraose as acceptor Caenorhabditis elegans

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+
-
 Caenorhabditis elegans

Organism

Organism UniProt Comment Textmining
Caenorhabditis elegans
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Caenorhabditis elegans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
GDP-fucose + Galbeta1-4GlcNAc-R
-
 Caenorhabditis elegans GDP + Galbeta1-4(Fucalpha1-3)GlcNAc-R Lewis x determinant ?
GDP-fucose + GalNAcbeta1-4GlcNAcbeta1-3Galbeta1-4Glc incubation with extracts from transfected COS7 cells Caenorhabditis elegans GDP + GalNAcbeta1-4[Fucalpha1-3]GlcNAcbeta1-3Galbeta1-4Glc product obtained at a level of about 50% of that obtained with the acceptor GalNAcbeta1-4GlcNAcbeta1-R ?
GDP-fucose + GalNAcbeta1-4GlcNAcbeta1-R
-
 Caenorhabditis elegans GDP + GalNAcbeta1-4[Fucalpha1-3]GlcNAcbeta1-R
-
 ?
GDP-fucose + lacto-N-neotetraose
-
 Caenorhabditis elegans GDP + lacto-N-fucopentaose III
-
 ?
GDP-fucose + NeuAcalpha2-3Galbeta1-4GlcNAc
-
 Caenorhabditis elegans GDP + NeuAcalpha2-3Galbeta1-4(Fucalpha1-3)GlcNAc sialyl Lewis x ?
additional information the enzyme has acceptor specificity properties similar to that of the human myeloid enzyme FTIV Caenorhabditis elegans ?
-
 ?
additional information the recombinant enzyme has a clear preference for nonsialylated type-2 acceptors over either neutral type-1 acceptors or sialylated type-2 acceptors Caenorhabditis elegans ?
-
 ?