Protein Variants | Comment | Organism |
---|---|---|
A349D | mutant enzyme shows higher activity with a range of acceptor substrates, higher affinity for Fucalpha(1,2)Galbeta(1,4)GlcNAc, 8fold higher overall catalytic efficiency than that of wild-type enzyme. The single amino acid site Asp336 of FucT III and Ala349 of FucT V constitutes the only difference in the sequence of FucT III and V over the final 210 COOH-terminal amino acid residues, impacts the acceptor substrate profiles of FucT III and FuvT V | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0188 | - |
GDP-L-fucose | fucosyltransferase V | Homo sapiens | |
0.0233 | - |
GDP-L-fucose | fucosyltransferase V mutant enzyme A349D | Homo sapiens | |
1.1 | - |
Fucalpha(1,2)Galbeta(1,4)GlcNAc | fucosyltransferase V mutant enzyme A349D | Homo sapiens | |
3.9 | - |
Fucalpha(1,2)Galbeta(1,4)GlcNAc | fucosyltransferase V | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
fucosyltransferase V | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
GDP-L-fucose + Fucalpha(1,2)Galbeta(1,4)GlcNAc | mutant enzyme A349D shows 8fold higher activity than the wild-type enzyme | Homo sapiens | GDP + Fucalpha(1,2)Galbeta(1,4)(Fucalpha(1,3))GlcNAc | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.7 | - |
Fucalpha(1,2)Galbeta(1,4)GlcNAc | fucosyltransferase V | Homo sapiens | |
3.6 | - |
Fucalpha(1,2)Galbeta(1,4)GlcNAc | fucosyltransferase V mutant enzyme A349D | Homo sapiens |