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Literature summary for 2.4.1.152 extracted from

  • Vo, L.; Lee, S.; Marcinko, M.C.; Holmes, E.H.; Macher, B.A.
    Human alpha1,3/4-fucosyltransferases II. A single amino acid at the COOH terminus of FucT III and V alters their kinetic properties (1998), J. Biol. Chem., 273, 25250-25255.
    View publication on PubMed

Protein Variants

Protein Variants Comment Organism
A349D mutant enzyme shows higher activity with a range of acceptor substrates, higher affinity for Fucalpha(1,2)Galbeta(1,4)GlcNAc, 8fold higher overall catalytic efficiency than that of wild-type enzyme. The single amino acid site Asp336 of FucT III and Ala349 of FucT V constitutes the only difference in the sequence of FucT III and V over the final 210 COOH-terminal amino acid residues, impacts the acceptor substrate profiles of FucT III and FuvT V Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0188
-
GDP-L-fucose fucosyltransferase V Homo sapiens
0.0233
-
GDP-L-fucose fucosyltransferase V mutant enzyme A349D Homo sapiens
1.1
-
Fucalpha(1,2)Galbeta(1,4)GlcNAc fucosyltransferase V mutant enzyme A349D Homo sapiens
3.9
-
Fucalpha(1,2)Galbeta(1,4)GlcNAc fucosyltransferase V Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
fucosyltransferase V
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
GDP-L-fucose + Fucalpha(1,2)Galbeta(1,4)GlcNAc mutant enzyme A349D shows 8fold higher activity than the wild-type enzyme Homo sapiens GDP + Fucalpha(1,2)Galbeta(1,4)(Fucalpha(1,3))GlcNAc
-
?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.7
-
Fucalpha(1,2)Galbeta(1,4)GlcNAc fucosyltransferase V Homo sapiens
3.6
-
Fucalpha(1,2)Galbeta(1,4)GlcNAc fucosyltransferase V mutant enzyme A349D Homo sapiens