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Literature summary for 2.4.1.152 extracted from

  • Johnson, P.H.; Donald, A.S.; Clarke, J.L.; Watkins, W.M.
    Purification, properties and possible gene assignment of an alpha 1,3-fucosyltransferase expressed in human liver (1995), Glycoconjugate J., 12, 879-893.
    View publication on PubMed
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KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0026
-
 N-acetyllactosamine pH 7.3, 37°C Homo sapiens
0.9
-
 NeuAcalpha(2,3)Galbeta(1,4)GlcNAc pH 7.3, 37°C Homo sapiens
1.3
-
 Fucalpha(1,2)Galbeta(1,4)GlcNAc
-
 Homo sapiens
1.5
-
 Galbeta(1,4)GlcNAcbeta(1,3)Galbeta(1,4)Glc pH 7.3, 37°C Homo sapiens
2.9
-
 Galbeta(1,4)GlcNAc pH 7.3, 37°C Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ activates, Km: 16 mM Homo sapiens
Mg2+ activates, Km: 22 mM Homo sapiens
Mn2+ activates, Km: 12 mM Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
41000
-
 gel filtration Homo sapiens
44000
-
 1 * 44000, SDS-PAGE Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
Hep-G2 cell
-
 Homo sapiens
-
liver
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.4
-
-
 Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
GDP-L-fucose + alpha(2,3)-sialyllactosamine 115% of the activity with Galbeta(1,4)GlcNAc Homo sapiens GDP + ?
-
 ?
GDP-L-fucose + Fucalpha(1,2)Galbeta(1,4)GlcNAc
-
 Homo sapiens GDP + Fucalpha(1,2)Galbeta(1,4)(Fucalpha(1,3))GlcNAc
-
 ?
GDP-L-fucose + Galbeta(1,4)GlcNAc i.e. N-acetyllactosamine Homo sapiens GDP + Galbeta(1,4)(Fucalpha(1,3))GlcNAc
-
 ?
GDP-L-fucose + Galbeta(1,4)GlcNAcbeta(1,3)Galbeta(1,4)Glc
-
 Homo sapiens GDP + Galbeta(1,4)(Fucalpha(1,3))GlcNAcbeta(1,3)Galbeta(1,4)Glc
-
 ?
GDP-L-fucose + NeuAcalpha(2,3)Galbeta(1,4)GlcNAc
-
 Homo sapiens GDP + NeuAcalpha(2,3)Galbeta(1,4)(Fucalpha(1,3))GlcNAc
-
 ?
additional information acceptor specificity with glycoprotein and glycolipid acceptors Homo sapiens ?
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 44000, SDS-PAGE Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7 8 reaction with N-acetyllactosamine Homo sapiens

pH Range

pH Minimum pH Maximum Comment Organism
6 8.8 pH 6.0: about 40% of maximal activity, pH 8.8: about 50% of maximal activity, reaction with N-acetyllactosamine Homo sapiens