Application | Comment | Organism |
---|---|---|
synthesis | stable expression of beta1,4-N-acetylgalactosaminyltransferase from Caenorhabditis elegans and human FucT9 in CHO cells, producing fucosylated poly-LacdiNAc N-glycans | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
expression in CHO cells | Homo sapiens |
poly-LDN backbone is efficiently fucosylated by recombinant human alpha1,3-fucosyltransferase IX (FucT 9) when it is stably co-expressed with the Cebeta4GalNAcT in cell line L8-GalNAcT-FucT | Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
MnCl2 | glycosyltransferase assay with 20 mM MnCl2 | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
GDP-fucose + (-3GalNAcbeta1-4GlcNAcbeta1-)n | - |
Homo sapiens | GDP + (-3GalNAcbeta1-4(Fucalpha1-3)GlcNAcbeta1-)n | N-glycans containing poly-LDN structures are efficiently fucosylated by human FucT9 producing poly-LDNF structures, max. 4-6 repeating LDNF units | ? | |
GDP-fucose + GalNAcbeta1-4GlcNAcbeta-R | - |
Homo sapiens | GDP + GalNAcbeta1-4(Fucalpha1-3)GlcNAcbeta1-R | - |
? |
Synonyms | Comment | Organism |
---|---|---|
alpha1,3-fucosyltransferase IX | - |
Homo sapiens |
FucT 9 | - |
Homo sapiens |
FucT9 | - |
Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
glycosyltransferase assay | Homo sapiens |