Cloned (Comment) | Organism |
---|---|
- |
Helicobacter pylori |
full-length and each truncated FucT (DELTAFucT) are expressed in Escherichia coli HMS174DE3 | Helicobacter pylori |
Protein Variants | Comment | Organism |
---|---|---|
additional information | with full length 11639FucT, 28% of total enzyme activities is localized in the soluble fraction. For alpha-(1,3/1,4)-fucosyltransferase(1441), this increases to 45%, respectively, indicating that truncation of the C-terminal putative alpha-helices increases FucT solubility. Truncation mutant alpha-(1,3/1,4)-fucosyltransferase(10447) completely loses activity | Helicobacter pylori |
additional information | with full length UA948FucT, 20% of total enzyme activities is localized in the soluble fraction. For UA948(1-428), this increases to 47%, respectively, indicating that truncation of the C-terminal putative alpha-helices increases FucT solubility. UA948(1-364), a mutant with the entire heptad repeat region removed, exhibits extremely low levels of enzyme activity. This indicates that the heptad repeat region is essential for enzyme activity. Construct alpha-(1,3/1,4)-fucosyltransferase(1-371) has little enzyme activity. alpha-(1,3/1,4)-fucosyltransferase(10-434) and alpha-(1,3/1,4)-fucosyltransferase(1-434) completely lose activity | Helicobacter pylori |
General Stability | Organism |
---|---|
lyophilized alpha-(1,3/1,4)-fucosyltransferase(1-428) loses 23% of its activity after 3 month | Helicobacter pylori |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0156 | - |
GDP-beta-L-fucose | cosubstrate: Galbeta1,4GlcNAc-O(CH2)8CO2CH3, truncated enzyme alpha-(1,3/1,4)-fucosyltransferase(1441) | Helicobacter pylori | |
0.0176 | - |
GDP-beta-L-fucose | cosubstrate: Galbeta1,4GlcNAc-O(CH2)8CO2CH3, truncated enzyme alpha-(1,3/1,4)-fucosyltransferase(1428) | Helicobacter pylori | |
0.0447 | - |
GDP-beta-L-fucose | cosubstrate: Galbeta1,4GlcNAc-O(CH2)8CO2CH3, full-length enzyme | Helicobacter pylori | |
0.056 | - |
GDP-beta-L-fucose | cosubstrate: Galbeta1,4GlcNAc-O(CH2)8CO2CH3, full-length enzyme | Helicobacter pylori | |
0.37 | - |
Galbeta1,4GlcNAc-O(CH2)8CO2CH3 | full-length enzyme | Helicobacter pylori | |
0.39 | - |
Galbeta1,4GlcNAc-O(CH2)8CO2CH3 | truncated enzyme alpha-(1,3/1,4)-fucosyltransferase(1441) | Helicobacter pylori | |
1.5 | - |
Galbeta1,4GlcNAc-O(CH2)8CO2CH3 | full-length enzyme | Helicobacter pylori | |
1.7 | - |
Galbeta1,4GlcNAc-O(CH2)8CO2CH3 | truncated enzyme alpha-(1,3/1,4)-fucosyltransferase(1428) | Helicobacter pylori |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
51200 | - |
x * 51200, alpha-(1,3/1,4)-fucosyltransferase(1-428), calculated from sequence | Helicobacter pylori |
51200 | - |
x * 51200, mutant alpha-(1,3/1,4)-fucosyltransferase (1441), calculated from sequence | Helicobacter pylori |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Helicobacter pylori | - |
- |
- |
Helicobacter pylori NCTC11639 | - |
- |
- |
Helicobacter pylori UA948 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Helicobacter pylori |
alpha-(1,3/1,4)-fucosyltransferase(1428) with a His6 tag at the C terminus is purified | Helicobacter pylori |
Storage Stability | Organism |
---|---|
lyophilized alpha-(1,3/1,4)-fucosyltransferase(1441) loses 38% activity after 3 months | Helicobacter pylori |
most stable at -80°C with or without glycerol supplementation | Helicobacter pylori |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
GDP-beta-L-fucose + 3-sulfo-Galbeta1,4GlcNAc | 135% of the activity with Galbeta1,4GlcNAc | Helicobacter pylori | ? | - |
? | |
GDP-beta-L-fucose + 3-sulfo-Galbeta1,4GlcNAc | 72% of the activity with Galbeta1,4GlcNAc | Helicobacter pylori | ? | - |
? | |
GDP-beta-L-fucose + 4-deoxy-Galbeta1,4GlcNAc | 8% of the activity with Galbeta1,4GlcNAc | Helicobacter pylori | ? | - |
? | |
GDP-beta-L-fucose + Fucalpha1,2Galbeta1,4GlcNAc | 115% of the activity with Galbeta1,4GlcNAc | Helicobacter pylori | ? | - |
? | |
GDP-beta-L-fucose + Fucalpha1,2Galbeta1,4GlcNAc | 99% of the activity with Galbeta1,4GlcNAc | Helicobacter pylori | ? | - |
? | |
GDP-beta-L-fucose + Galbeta1,4GlcNAc | - |
Helicobacter pylori | ? | - |
? | |
GDP-beta-L-fucose + NeuAcalpha2,3Galbeta1,4GlcNAc | 32% of the activity with Galbeta1,4GlcNAc | Helicobacter pylori | ? | - |
? | |
GDP-L-fucose + Galbeta(1,4)GlcNAc-O(CH2)8CO2CH3 | - |
Helicobacter pylori | GDP + Galbeta(1,4)(Fucalpha(1,3))GlcNAc-O(CH2)8CO2CH3 | - |
? | |
additional information | the enzyme shows alpha1,3- and alpha1,4-fucosyltransferase activity | Helicobacter pylori | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 51200, alpha-(1,3/1,4)-fucosyltransferase(1-428), calculated from sequence | Helicobacter pylori |
? | x * 51200, mutant alpha-(1,3/1,4)-fucosyltransferase (1441), calculated from sequence | Helicobacter pylori |
Synonyms | Comment | Organism |
---|---|---|
11639FucT | - |
Helicobacter pylori |
alpha-(1,3/1,4)-fucosyltransferase | - |
Helicobacter pylori |
alpha-(1,3/1,4)-FucT | - |
Helicobacter pylori |
UA948FucT | - |
Helicobacter pylori |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.1 | - |
GDP-beta-L-fucose | cosubstrate: Galbeta1,4GlcNAc-O(CH2)8CO2CH3, truncated enzyme alpha-(1,3/1,4)-fucosyltransferase(1428) | Helicobacter pylori | |
1.1 | - |
Galbeta1,4GlcNAc-O(CH2)8CO2CH3 | truncated enzyme alpha-(1,3/1,4)-fucosyltransferase(1428) | Helicobacter pylori | |
2.3 | - |
GDP-beta-L-fucose | cosubstrate: Galbeta1,4GlcNAc-O(CH2)8CO2CH3, truncated enzyme alpha-(1,3/1,4)-fucosyltransferase(1428) | Helicobacter pylori | |
2.3 | - |
Galbeta1,4GlcNAc-O(CH2)8CO2CH3 | truncated enzyme alpha-(1,3/1,4)-fucosyltransferase(1428) | Helicobacter pylori |