Cloned (Comment) | Organism |
---|---|
CEFT DNA and amino acid sequence determination and analysis, expression analysis, expression in COS-7 cells | Caenorhabditis elegans |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | CEFT-3 acts optimally in the presence of EDTA/EGTA, indicating that cations inhibit this activity | Caenorhabditis elegans | |
Ni2+ | inhibits CEFT-4 | Caenorhabditis elegans |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | CEFT-3 does not require divalent cations, while CEFT-4 is activates by some divalent cations, with slight prefernce for Ca2+ | Caenorhabditis elegans |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Caenorhabditis elegans | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | CEFT is N-glycosylated | Caenorhabditis elegans |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | developmental expression of CEFT isozymes, overview | Caenorhabditis elegans | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
GDP-beta-L-fucose + Galbeta1-4GlcNAcalpha-4-nitrophenol | preferred substrate of CEFT-4 and CEFT-3 | Caenorhabditis elegans | GDP + Galbeta1-4(Fucalpha1-3)GlcNAcalpha-4-nitrophenol | - |
? | |
GDP-beta-L-fucose + Galbeta1-4GlcNAcbeta1-3Galbeta1-4Glc | activity of CEFT-3 aqnd CEFT-4 | Caenorhabditis elegans | GDP + Galbeta1-4(Fucalpha1-3)GlcNAcbeta1-3Galbeta1-4Glc | - |
? | |
GDP-beta-L-fucose + Galbeta1-4GlcNAcbeta1-3Galbeta1-4Glc | substrate of CEFT-3 | Caenorhabditis elegans | GDP + Galbeta1-4(Fucalpha1-3)GlcNAcbeta1-3Galbeta1-4Glc | - |
? | |
GDP-beta-L-fucose + GalNAcbeta1-4GlcNAcbeta1-3Galbeta1-4Glc | activity of CEFT-2 | Caenorhabditis elegans | GDP + GalNAcbeta1-4(Fucalpha1-3)GlcNAcbeta1-3Galbeta1-4Glc | - |
? | |
additional information | acceptor specificity of recombinant CEFTs, CEFT-1 acts on the N-glycan pentasaccharide core acceptor Manalpha1-3(Manalpha1-6)Manbeta1-4GlcNAcbeta1-4GlcNAcbeta1-Asn, while CEFT-2 does not, overview | Caenorhabditis elegans | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
CEFT-3 | - |
Caenorhabditis elegans |
CEFT-4 | - |
Caenorhabditis elegans |
More | the enzyme belongs to the alpha1,3-fucosyltransferase family | Caenorhabditis elegans |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
22 | - |
optimal enzyme activity at room temperature, rapid loss of activity at 37°C | Caenorhabditis elegans |