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Literature summary for 2.4.1.152 extracted from

  • Lee, J.; Pandey, R.; Kim, D.; Sohng, J.
    Cloning and functional characterization of an alpha-1,3-fucosyltransferase from Bacteroides fragilis (2013), Biotechnol. Bioprocess Eng., 18, 843-849.
No PubMed abstract available
Search for more literature for 2.4.1.152

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Bacteroides fragilis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.12
-
 GDP-beta-L-fucose at pH 7.5 and 37°C Bacteroides fragilis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
38000
-
 x * 38000, SDS-PAGE Bacteroides fragilis
39000
-
 x * 39000, calculated from amino acid sequence Bacteroides fragilis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
GDP-beta-L-fucose + N-acetyllactosamine Bacteroides fragilis
-
 GDP + L-Fuc-alpha-(1->3)-[D-Gal-beta(1->4)]-D-GlcNac
-
 ?
GDP-beta-L-fucose + N-acetyllactosamine Bacteroides fragilis NCTC 9343
-
 GDP + L-Fuc-alpha-(1->3)-[D-Gal-beta(1->4)]-D-GlcNac
-
 ?

Organism

Organism UniProt Comment Textmining
Bacteroides fragilis
-
-
-
Bacteroides fragilis NCTC 9343
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
GDP-beta-L-fucose + N-acetyllactosamine
-
 Bacteroides fragilis GDP + L-Fuc-alpha-(1->3)-[D-Gal-beta(1->4)]-D-GlcNac
-
 ?
GDP-beta-L-fucose + N-acetyllactosamine
-
 Bacteroides fragilis NCTC 9343 GDP + L-Fuc-alpha-(1->3)-[D-Gal-beta(1->4)]-D-GlcNac
-
 ?

Subunits

Subunits Comment Organism
? x * 38000, SDS-PAGE Bacteroides fragilis
? x * 39000, calculated from amino acid sequence Bacteroides fragilis

Synonyms

Synonyms Comment Organism
alpha-1,3-fucosyltransferase 3
-
 Bacteroides fragilis
alpha-1,3-FucT3
-
 Bacteroides fragilis