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Literature summary for 2.4.1.155 extracted from

  • Sasai, K.; Ikeda, Y.; Fujii, T.; Tsuda, T.; Taniguchi, N.
    UDP-GlcNAc concentration is an important factor in the biosynthesis of beta1,6-branched oligosaccharides: Regulation based on the kinetic properties of N-acetylglucosaminyltransferase V (2002), Glycobiology, 12, 119-127.
    View publication on PubMed
Search for more literature for 2.4.1.155

Cloned(Commentary)

Cloned (Comment) Organism
fusion with signal peptide from baculoviral protein to permit secretion into medium, BVVdelta73 and BVV delta188 Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.13
-
 N-acetyl-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3(6)-(N-acetyl-D-glucosaminyl-1,2-alpha-D-mannosyl-1,6(3))-beta-D-mannosyl-1,4-N-acetyl-D-glucosaminyl-R with TDP-glucose Homo sapiens
0.15
-
 N-acetyl-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3(6)-(N-acetyl-D-glucosaminyl-1,2-alpha-D-mannosyl-1,6(3))-beta-D-mannosyl-1,4-N-acetyl-D-glucosaminyl-R with UDP-N-acetylglucosamine Homo sapiens
0.23
-
 N-acetyl-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3(6)-(N-acetyl-D-glucosaminyl-1,2-alpha-D-mannosyl-1,6(3))-beta-D-mannosyl-1,4-N-acetyl-D-glucosaminyl-R with UDP-glucose Homo sapiens
2.9
-
 UDP-glucose
-
 Homo sapiens
2.9
-
 TDP-glucose
-
 Homo sapiens
4
-
 UDP-N-acetylglucosamine BVVdelta188 mutant Homo sapiens
4.6
-
 UDP-N-acetylglucosamine BVVdelta73 mutant Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
soluble fusion with signal peptide from baculoviral protein to permit secretion into medium Homo sapiens
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
70000
-
 SDS-PAGE, BVVdelta188 Homo sapiens
85000
-
 SDS-PAGE, BVVdelta73 Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Homo sapiens

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0036
-
 BVVDELTA73 mutant Homo sapiens
0.004
-
 BVVDELTA188 mutant Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information ADP-glucose, CDP-glucose and GDP-glucose do not act as glycosyl donor Homo sapiens ?
-
 ?
TDP-glucose + N-acetyl-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3(6)-(N-acetyl-D-glucosaminyl-1,2-alpha-D-mannosyl-1,6(3))-beta-D-mannosyl-1,4-N-acetyl-D-glucosaminyl-R
-
 Homo sapiens TDP + ?
-
 ?
UDP-glucose + N-acetyl-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3(6)-(N-acetyl-D-glucosaminyl-1,2-alpha-D-mannosyl-1,6(3))-beta-D-mannosyl-1,4-N-acetyl-D-glucosaminyl-R
-
 Homo sapiens UDP + ?
-
 ?
UDP-N-acetyl-D-glucosamine + N-acetyl-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3(6)-(N-acetyl-D-glucosaminyl-1,2-alpha-D-mannosyl-1,6(3))-beta-D-mannosyl-1,4-N-acetyl-D-glucosaminyl-R
-
 Homo sapiens UDP + N-acetyl-D-glucosaminyl-1,2-(N-acetyl-D-glucosaminyl-1,6)-1,2-alpha-D-mannosyl-1,3(6)-(N-acetyl-D-glucosaminyl-1,2-alpha-D-mannosyl-1,6(3))-beta-D-mannosyl-1,4-N-acetyl-D-glucosaminyl-R
-
 ?