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Literature summary for 2.4.1.160 extracted from
- Specificity of a particulate glucosyltransferase in seedlings of Pisum sativum L. which catalyzes the formation of 5-O-(beta-D-glucopyranosyl)pyridoxine (1982), J. Nutr. Sci. Vitaminol., 28, 359-366.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 4'-deoxypyridoxine | competitive inhibitor of pyridoxamine and pyridoxine glucosylation | Pisum sativum |  |
| pyridoxamine | competitive inhibitor of pyridoxine glucosylation | Pisum sativum | |
| pyridoxine | competitive inhibitor of pyridoxamine glucosylation | Pisum sativum | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.25 | - |
pyridoxine | - |
Pisum sativum | |
| 0.26 | - |
pyridoxamine | - |
Pisum sativum | |
| 0.67 | - |
UDPglucose | cosubstrate pyridoxine | Pisum sativum | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | required, Km: 1.0 mM | Pisum sativum | |
| Mn2+ | can replace Mg2+, 40% as effective as Mg2+ | Pisum sativum | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| UDPglucose + pyridoxine | Pisum sativum | - |
UDP + 5'-O-beta-D-glucosylpyridoxine | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pisum sativum | - |
podded pea, L. cv. Kinusaya | - |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| seedling | - |
Pisum sativum | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| UDPglucose + 4'-deoxypyridoxine | - |
Pisum sativum | UDP + 5'-O-beta-D-glucosyl-4'-deoxypyridoxine | - |
? | |
| UDPglucose + pyridoxamine | - |
Pisum sativum | UDP + 5'-O-beta-D-glucosylpyridoxamine | - |
? | |
| UDPglucose + pyridoxine | - |
Pisum sativum | UDP + 5'-O-beta-D-glucosylpyridoxine | - |
? | |
| UDPglucose + pyridoxine | high specificity for UDPglucose | Pisum sativum | UDP + 5'-O-beta-D-glucosylpyridoxine | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Pisum sativum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.8 | 8.8 | pyridoxine + UDPglucose | Pisum sativum |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 9.5 | about 50% of maximum activity at pH 7 and 9.5 | Pisum sativum |
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