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Literature summary for 2.4.1.17 extracted from
- Novel polymorphic human UDP-glucuronosyltransferase 2A3: cloning, functional characterization of enzyme variants, comparative tissue expression, and gene induction (2008), Mol. Pharmacol., 74, 744-754.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| additional information | rifampicin increases the UGT2A3 mRNA by more than 4.5-fold n LS180 intestinal cells, no or poor induction by dexamethasone or beta-naphthoflavone | Homo sapiens |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene UGT2A3, located on chromosome 4q13, DNA and amino acid sequence determination and analysis, the UGT2A3 proximal promoter contains putative HNF1alpha, CDX2, PXR, and GR consensus elements, sequence comparisons, expression of UGT2A3 in Spodoptera frugiperda SF21 cells using the baculovirus transfection system | Homo sapiens |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| T497A | naturally occuring polymorphism, approximately 10% of clones contains a A1489G nucleotide substitution, yielding proteins with a residue 497 alanine in UGT2A3.2 instead of a threonine in UGT2A3.1. The allele frequency of this polymorphism rs13128286 was 0.13 in a European-American population as determined by direct DNA sequencing, UGT2A3 allozymes vary in hyodeoxycholic acid glucuronidation kinetics, overview | Homo sapiens |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.044 | - |
hyodeoxycholic acid | recombinant genetic variant UGT2A3.2 | Homo sapiens |  |
| 0.069 | - |
hyodeoxycholic acid | recombinant genetic variant UGT2A3.1 | Homo sapiens | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| microsome | - |
Homo sapiens | - |
- |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| UDP-D-glucuronate + acceptor | Homo sapiens | - |
UDP + acceptor beta-D-glucuronoside | - |
? | |
| UDP-D-glucuronate + chenodeoxycholic acid | Homo sapiens | isozyme UGT2A3 | UDP + hyodeoxycholic acid 6-O-glucuronoside | - |
? | |
| UDP-D-glucuronate + deoxycholic acid | Homo sapiens | isozyme UGT2A3 | UDP + hyodeoxycholic acid 6-O-glucuronoside | - |
? | |
| UDP-D-glucuronate + hyodeoxycholic acid | Homo sapiens | isozyme UGT2A3 | UDP + hyodeoxycholic acid 6-O-glucuronoside | - |
? | |
| UDP-D-glucuronate + ursodeoxycholic acid | Homo sapiens | isozyme UGT2A3 | UDP + hyodeoxycholic acid 6-O-glucuronoside | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | Q6UWM9 | isozyme UGT2A3 | - |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| adipose tissue | expression level is 91% of the expression level in liver | Homo sapiens | - |
| colon | expression level is 78% of the expression level in liver | Homo sapiens | - |
| liver | - |
Homo sapiens | - |
| LS-180 cell | intestinal cell line | Homo sapiens | - |
| additional information | isozyme UGT2A3 expression analysis in human tissues, overview | Homo sapiens | - |
| small intestine | expression level is 160% of the expression level in liver | Homo sapiens | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | no activity of UGT2A3 with 4-nitrophenol, 4-methylumbelliferone, or any other small phenolic substrate | Homo sapiens | ? | - |
? | |
| UDP-D-glucuronate + acceptor | - |
Homo sapiens | UDP + acceptor beta-D-glucuronoside | - |
? | |
| UDP-D-glucuronate + acceptor | the recombinant isozyme 2A3, expressed in insect cells via baculovirus system, selectively glucuronidates bile acids, particularly hyodeoxycholic acid at the 6-hydroxy position, wide substrate specificity, detailed overview | Homo sapiens | UDP + acceptor beta-D-glucuronoside | - |
? | |
| UDP-D-glucuronate + chenodeoxycholic acid | isozyme UGT2A3 | Homo sapiens | UDP + hyodeoxycholic acid 6-O-glucuronoside | - |
? | |
| UDP-D-glucuronate + deoxycholic acid | isozyme UGT2A3 | Homo sapiens | UDP + hyodeoxycholic acid 6-O-glucuronoside | - |
? | |
| UDP-D-glucuronate + hyodeoxycholic acid | isozyme UGT2A3 | Homo sapiens | UDP + hyodeoxycholic acid 6-O-glucuronoside | - |
? | |
| UDP-D-glucuronate + hyodeoxycholic acid | recombinant enzyme, expressed in insect cells via baculovirus system, selective glucuronidation | Homo sapiens | UDP + hyodeoxycholic acid 6-O-glucuronoside | product formation depends on the isozyme, overview | ? | |
| UDP-D-glucuronate + hyodeoxycholic acid | recombinant enzyme, expressed in insect cells via baculovirus system, selective glucuronidation | Homo sapiens | UDP + hyodeoxycholic acid 24-carboxy-glucuronoside | product formation depends on the isozyme, overview | ? | |
| UDP-D-glucuronate + hyodeoxycholic acid | recombinant enzyme, expressed in insect cells via baculovirus system, selective glucuronidation | Homo sapiens | UDP + hyodeoxycholic acid 3-O-glucuronoside | product formation depends on the isozyme, overview | ? | |
| UDP-D-glucuronate + ursodeoxycholic acid | isozyme UGT2A3 | Homo sapiens | UDP + hyodeoxycholic acid 6-O-glucuronoside | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| UDP-glucuronosyltransferase 2A3 | - |
Homo sapiens |
| UGT2A3 | - |
Homo sapiens |
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