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Literature summary for 2.4.1.17 extracted from

  • Rennie, E.A.; Ebert, B.; Miles, G.P.; Cahoon, R.E.; Christiansen, K.M.; Stonebloom, S.; Khatab, H.; Twell, D.; Petzold, C.J.; Adams, P.D.; Dupree, P.; Heazlewood, J.L.; Cahoon, E.B.; Scheller, H.V.
    Identification of a sphingolipid alpha-glucuronosyltransferase that is essential for pollen function in Arabidopsis (2014), Plant Cell, 26, 3314-3325 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.4.1.17

Cloned(Commentary)

Cloned (Comment) Organism
gene IPUT1, functional enzyme overexpression in Saccharomyces cerevisiae, coexpression with a human UDP-GlcA transporter, GIPCs from Saccharomyces cerevisiae do not contain GlcA. Production of GlcA-IPC in yeast Arabidopsis thaliana
IPUT1 overexpression in Saccharomyces Nicotiana benthamiana using the Agrobacterium tumefaciens strain C58-1 transfection method, coexpression with a glucuronosyltransferase, GUX1. Total glucosylceramide levels decrease slightly compared with the p19 control, while levels of GlcN-GlcA-IPC increase Nicotiana benthamiana

Protein Variants

Protein Variants Comment Organism
additional information introduction of Arabdiopsis thaliana IPUT1 gene together with genes for a UDP-glucose dehydrogenase from Arabidopsis thaliana and a human UDP-GlcA transporter into a yeast mutant deficient in the endogenous inositol phosphorylceramide (IPC) mannosyltransferase. In this engineered yeast strain, IPUT1 transfers GlcA to IPC Arabidopsis thaliana
additional information overexpression or silencing of IPUT1 in Nicotiana benthamiana results in an increase or a decrease, respectively, in IPC glucuronosyltransferase activity in vitro. Plants in which IPUT1 is silenced accumulate IPC, the immediate precursor, as well as ceramides and glucosylceramides. Plants overexpressing IPUT1 show an increased content of GIPCs. Mutations in IPUT1 are not transmitted through pollen, indicating that these sphingolipids are essential in plants. Silencing of the Nicotiana benthamiana IPUT1 homologue results in a reduction of the [14C]GlcA-IPC band to about 35% of that produced by GUS-silenced control microsomes Nicotiana benthamiana

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
 Nicotiana benthamiana 16020
-
membrane
-
 Arabidopsis thaliana 16020
-
microsome
-
 Nicotiana benthamiana
-
-
microsome
-
 Arabidopsis thaliana
-
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
UDP-glucuronate + acceptor Nicotiana benthamiana transfer of glucuronic acid (GlcA) from UDP-GlcA to glycosyl inositol phosphorylceramides (GIPCs) UDP + acceptor beta-D-glucuronoside
-
 ?
UDP-glucuronate + acceptor Arabidopsis thaliana transfer of glucuronic acid (GlcA) from UDP-GlcA to glycosyl inositol phosphorylceramides (GIPCs) UDP + acceptor beta-D-glucuronoside
-
 ?

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana Q8GWB7
-
-
Nicotiana benthamiana
-
 cv. Domin
-

Source Tissue

Source Tissue Comment Organism Textmining
pollen
-
 Nicotiana benthamiana
-
pollen
-
 Arabidopsis thaliana
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
UDP-glucuronate + acceptor transfer of glucuronic acid (GlcA) from UDP-GlcA to glycosyl inositol phosphorylceramides (GIPCs) Nicotiana benthamiana UDP + acceptor beta-D-glucuronoside
-
 ?
UDP-glucuronate + acceptor transfer of glucuronic acid (GlcA) from UDP-GlcA to glycosyl inositol phosphorylceramides (GIPCs) Arabidopsis thaliana UDP + acceptor beta-D-glucuronoside
-
 ?

Synonyms

Synonyms Comment Organism
inositol phosphorylceramide glucuronosyltransferase1
-
 Nicotiana benthamiana
inositol phosphorylceramide glucuronosyltransferase1
-
 Arabidopsis thaliana
IPUT1
-
 Nicotiana benthamiana
IPUT1
-
 Arabidopsis thaliana

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
21
-
 assay at Nicotiana benthamiana
21
-
 assay at Arabidopsis thaliana

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 assay at Nicotiana benthamiana
7
-
 assay at Arabidopsis thaliana

General Information

General Information Comment Organism
evolution IPUT1 belongs to a glycosyltransferase family that transfers sugars with a retaining mechanism resulting in alpha-linked sugars, consistent with the alpha conformation of the GlcA in glycosyl inositol phosphorylceramides (GIPCs) Nicotiana benthamiana
evolution IPUT1 belongs to a glycosyltransferase family that transfers sugars with a retaining mechanism resulting in alpha-linked sugars, consistent with the alpha conformation of the GlcA in glycosyl inositol phosphorylceramides (GIPCs) Arabidopsis thaliana
malfunction overexpression or silencing of IPUT1 in Nicotiana benthamiana results in an increase or a decrease, respectively, in IPC glucuronosyltransferase activity in vitro. Plants in which IPUT1 is silenced accumulate IPC, the immediate precursor, as well as ceramides and glucosylceramides. Plants overexpressing IPUT1 show an increased content of GIPCs. Mutations in IPUT1 are not transmitted through pollen, indicating that these sphingolipids are essential in plants Nicotiana benthamiana
metabolism the enzyme is involved in the plant-type sphingolipid pathway Nicotiana benthamiana
metabolism the enzyme is involved in the plant-type sphingolipid pathway Arabidopsis thaliana
physiological function inositol phosphorylceramide glucuronosyltransferase1 (IPUT1) transfers GlcA from UDP-GlcA to glycosyl inositol phosphorylceramides (GIPCs) Nicotiana benthamiana
physiological function inositol phosphorylceramide glucuronosyltransferase1 (IPUT1) transfers GlcA from UDP-GlcA to glycosyl inositol phosphorylceramides (GIPCs) Arabidopsis thaliana