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Literature summary for 2.4.1.170 extracted from
- UDP-glucose:isoflavone 7-O-glucosyltransferase from roots of chick pea (Cicer arietinum L.) (1981), Arch. Biochem. Biophys., 212, 98-104.
General Stability
| General Stability | Organism |
|---|---|
| 2-mercaptoethanol, 40 mM, stabilizes during purification | Cicer arietinum |
| dithioerythritol, 10 mM, effectively stabilizes during purification | Cicer arietinum |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.012 | - |
Biochanin A | - |
Cicer arietinum |  |
| 0.024 | - |
formononetin | - |
Cicer arietinum | |
| 0.2 | - |
UDPglucose | - |
Cicer arietinum | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 42000 | - |
sucrose density gradient ultracentrifugation | Cicer arietinum |
| 50000 | - |
gel filtration | Cicer arietinum |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Cicer arietinum | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Cicer arietinum |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| root | - |
Cicer arietinum | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Cicer arietinum |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, 20% v/v glycerol, stable for several months | Cicer arietinum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| UDP-glucose + biochanin A | - |
Cicer arietinum | UDP + biochanin A 7-O-beta-D-glucoside | - |
? | |
| UDP-glucose + daidzein | poor substrate | Cicer arietinum | UDP + daidzein 7-O-beta-D-glucoside | - |
? | |
| UDP-glucose + formononetin | - |
Cicer arietinum | UDP + formononetin 7-O-beta-D-glucoside | - |
? | |
| UDP-glucose + genistein | poor substrate | Cicer arietinum | UDP + genistein 7-O-beta-D-glucoside | - |
? | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.5 | 9 | - |
Cicer arietinum |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 8.5 | 9 | optimal stability | Cicer arietinum |
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Release 2023.1 (January 2023)
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