Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.4.1.170 extracted from

  • Kim, J.H.; Kim, B.G.; Park, Y.; Ko, J.H.; Lim, C.E.; Lim, J.; Lim, Y.; Ahn, J.
    Characterization of flavonoid 7-O-glucosyltransferase from Arabidopsis thaliana (2006), Biosci. Biotechnol. Biochem., 70, 1471-1477.
    View publication on PubMed
Search for more literature for 2.4.1.170

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Arabidopsis thaliana

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.018
-
 eriodictyol
-
 Arabidopsis thaliana
0.0263
-
 kaempferol
-
 Arabidopsis thaliana
0.0353
-
 quercetin
-
 Arabidopsis thaliana

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana Q8VZE9
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Arabidopsis thaliana

Source Tissue

Source Tissue Comment Organism Textmining
flower AtGT-2 is expressed in this tissue, higher in the flowers and leaves than in roots and stems Arabidopsis thaliana
-
leaf AtGT-2 is expressed in this tissue, higher in the flowers and leaves than in roots and stems Arabidopsis thaliana
-
root AtGT-2 is expressed in this tissue, higher in the flowers and leaves than in roots and stems Arabidopsis thaliana
-
stem AtGT-2 is expressed in this tissue, higher in the flowers and leaves than in roots and stems Arabidopsis thaliana
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 Vmax for eriodictyol is 333.3 pkat/mg, 333.3 pkat/mg for kaempferol and 250 pkat/mg for quercetin. Assay at pH 7.0 and 37°C Arabidopsis thaliana

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information caffeic acid and esculetin do not react with the enzyme. AtGT-2 prefers flavonene to flavone or flavonol, both of which contain a double bond in the C ring. The relative conversion rate is 100% for eriodictyol, 71.6% for naringenin, 29.6% for apigenin, 92.1% for luteolin, 78.9% for kaempferol and 64.1% for quercetin. Arabidopsis thaliana ?
-
 ?
UDP-glucose + eriodictyol
-
 Arabidopsis thaliana UDP + eriodictyol 7-O-beta-D-glucoside
-
 ?
UDP-glucose + kaempferol
-
 Arabidopsis thaliana UDP + kaempferol 7-O-beta-D-glucoside
-
 ?
UDP-glucose + quercetin
-
 Arabidopsis thaliana UDP + quercetin 7-O-beta-D-glucoside
-
 ?

Synonyms

Synonyms Comment Organism
AtGT-2 AtGT-2 encodes a flavonoid 7-O-glucosyltransferase Arabidopsis thaliana

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
additional information
-
 additional information Kcat/Km for eriodictyol is 2.66 microM-1/mg-1, 1.32 microM-1/mg-1 for kaempferol and 250 microM-1/mg-1 for quercetin. Assay at pH 7.0 and 37°C Arabidopsis thaliana