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Literature summary for 2.4.1.173 extracted from
- Expression of UDP-glucose: poriferasterol glucosyltransferase in the process of differentiation of a true slime mold, Physarum polycephalum (1989), Biochim. Biophys. Acta, 992, 412-415.
Application
| Application | Comment | Organism |
|---|---|---|
| synthesis | synthesis of poriferasterol monoglucoside may be involved in differentiation | Physarum polycephalum |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0048 | - |
poriferasterol | - |
Physarum polycephalum |  |
| 0.12 | - |
UDP-glucose | - |
Physarum polycephalum | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| membrane | - |
Physarum polycephalum | 16020 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 72000 | - |
gel filtration | Physarum polycephalum |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| UDP-glucose + poriferasterol | Physarum polycephalum | synthesis of poriferasterol monoglucoside may be involved in differentiation | UDP + poriferasterol 3-beta-glucoside | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Physarum polycephalum | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial purification by differential centrifugation and gel filtration on Sephadex G-100 | Physarum polycephalum |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| cell culture | strain F and J activity only in diploid cells | Physarum polycephalum | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| UDP-glucose + campesterol | 80% of the activity with poriferasterol | Physarum polycephalum | UDP + campesterol 3-beta-D-glucoside | - |
? | |
| UDP-glucose + cholesterol | 12% of the activity with poriferasterol | Physarum polycephalum | UDP + cholesterol 3-beta-D-glucoside | - |
? | |
| UDP-glucose + ergosterol | 9% of the activity with poriferasterol | Physarum polycephalum | UDP + ergosterol 3-beta-D-glucoside | - |
? | |
| UDP-glucose + poriferasterol | synthesis of poriferasterol monoglucoside may be involved in differentiation | Physarum polycephalum | UDP + poriferasterol 3-beta-glucoside | - |
? | |
| UDP-glucose + sitosterol | maximal activity, the same as poriferasterol | Physarum polycephalum | UDP + sitosterol 3-beta-D-glucoside | - |
? | |
| UDP-glucose + stigmasterol | 14% of the activity with poriferasterol | Physarum polycephalum | UDP + stigmasterol 3-beta-D-glucoside | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Physarum polycephalum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
- |
Physarum polycephalum |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 7.2 | - |
Tris-HCl 0.1 M | Physarum polycephalum |
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Release 2023.1 (January 2023)
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