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Literature summary for 2.4.1.173 extracted from

  • Warnecke, D.C.; Baltrusch, M.; Buck, F.; Wolter, F.P.; Heinz, E.
    UDP-glucose:sterol glucosyltransferase: cloning and functional expression in Escherichia coli (1997), Plant Mol. Biol., 35, 597-603.
    View publication on PubMed
Search for more literature for 2.4.1.173

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Arabidopsis thaliana
expression in Escherichia coli Avena sativa

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
66000
-
 cloned, N-terminal peptide of 133 amino acid could be cleaved resulting in a molecular weight of 52000 in agreement with 56000 of the purified enzyme Avena sativa
69000
-
 cloned, calculated molecular mass Arabidopsis thaliana

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana Q9M8Z7 ugt80A2
-
Avena sativa O22678 ugt80A1
-
Avena sativa ugt80A1 O22678 ugt80A1
-

Source Tissue

Source Tissue Comment Organism Textmining
flower cDNA library Arabidopsis thaliana
-
leaf cDNA library Arabidopsis thaliana
-
root cDNA library Arabidopsis thaliana
-
shoot cDNA library Arabidopsis thaliana
-
shoot cloned from Avena sativa
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
UDP-glucose + cholesterol cloned enzyme exhibits equal activity to the purified protein Arabidopsis thaliana UDP + cholesterol 3-beta-D-glucoside
-
 ?
UDP-glucose + cholesterol cloned enzyme exhibits equal activity to the purified protein Avena sativa UDP + cholesterol 3-beta-D-glucoside
-
 ?
UDP-glucose + cholesterol cloned enzyme exhibits equal activity to the purified protein Avena sativa ugt80A1 UDP + cholesterol 3-beta-D-glucoside
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Arabidopsis thaliana
30
-
 assay at Avena sativa

pH Stability

pH Stability pH Stability Maximum Comment Organism
8
-
-
 Arabidopsis thaliana
8
-
 Tris-HCl 100 mM, dithiothreitol 1 mM, Triton X-100 0.2% Avena sativa