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Literature summary for 2.4.1.177 extracted from

  • Schulenburg, K.; Feller, A.; Hoffmann, T.; Schecker, J.H.; Martens, S.; Schwab, W.
    Formation of beta-glucogallin, the precursor of ellagic acid in strawberry and raspberry (2016), J. Exp. Bot., 67, 2299-2308 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.4.1.177

Application

Application Comment Organism
nutrition GT2 enzymes might contribute to the production of ellagic acid/ellagitannins in strawberry and raspberry, and are useful to develop strawberry fruit with additional health benefits and for the biotechnological production of bioactive polyphenols Fragaria x ananassa
nutrition GT2 enzymes might contribute to the production of ellagic acid/ellagitannins in strawberry and raspberry, and are useful to develop strawberry fruit with additional health benefits and for the biotechnological production of bioactive polyphenols Fragaria vesca
nutrition GT2 enzymes might contribute to the production of ellagic acid/ellagitannins in strawberry and raspberry, and are useful to develop strawberry fruit with additional health benefits and for the biotechnological production of bioactive polyphenols Rubus idaeus
synthesis GT2 enzymes might contribute to the production of ellagic acid/ellagitannins in strawberry and raspberry, and are useful to develop strawberry fruit with additional health benefits and for the biotechnological production of bioactive polyphenols Fragaria x ananassa
synthesis GT2 enzymes might contribute to the production of ellagic acid/ellagitannins in strawberry and raspberry, and are useful to develop strawberry fruit with additional health benefits and for the biotechnological production of bioactive polyphenols Fragaria vesca
synthesis GT2 enzymes might contribute to the production of ellagic acid/ellagitannins in strawberry and raspberry, and are useful to develop strawberry fruit with additional health benefits and for the biotechnological production of bioactive polyphenols Rubus idaeus

Cloned(Commentary)

Cloned (Comment) Organism
gene GT2, recombinant expression of GST-tagged enzyme in Escherichia coli strain BL21(DE3) pLysS Fragaria x ananassa
gene GT2, recombinant expression of GST-tagged enzyme in Escherichia coli strain BL21(DE3) pLysS Fragaria vesca
gene GT2, recombinant expression of GST-tagged enzyme in Escherichia coli strain BL21(DE3) pLysS Rubus idaeus
gene GT5, recombinant expression of GST-tagged enzyme in Escherichia coli strain BL21(DE3) pLysS Fragaria x ananassa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.035
-
 sinapate recombinant enzyme, pH 7.0, 30°C Fragaria x ananassa
0.045
-
 sinapate recombinant enzyme, pH 7.0, 30°C Fragaria x ananassa
0.08
-
 gallate recombinant enzyme, pH 7.0, 30°C Rubus idaeus
0.094
-
 vanillate recombinant enzyme, pH 7.0, 30°C Fragaria x ananassa
0.096
-
 gallate recombinant enzyme, pH 7.0, 30°C Fragaria x ananassa
0.232
-
 gallate recombinant enzyme, pH 7.0, 30°C Fragaria vesca
0.262
-
 syringate recombinant enzyme, pH 7.0, 30°C Fragaria x ananassa
0.315
-
 4-hydroxybenzoate recombinant enzyme, pH 7.0, 30°C Fragaria x ananassa
0.318
-
 UDP-glucose recombinant enzyme, pH 7.0, 30°C Fragaria vesca
0.364
-
 vanillate recombinant enzyme, pH 7.0, 30°C Fragaria x ananassa
0.378
-
 UDP-glucose recombinant enzyme, pH 7.0, 30°C Fragaria x ananassa
0.417
-
 UDP-glucose recombinant enzyme, pH 7.0, 30°C Rubus idaeus
0.515
-
 4-hydroxybenzoate recombinant enzyme, pH 7.0, 30°C Fragaria x ananassa
0.518
-
 syringate recombinant enzyme, pH 7.0, 30°C Fragaria x ananassa
0.529
-
 UDP-glucose recombinant enzyme, pH 7.0, 30°C Fragaria x ananassa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
UDP-glucose + 2-coumarate Fragaria x ananassa
-
 UDP + 2-coumaroyl beta-D-glucoside
-
 ?
UDP-glucose + 2-coumarate Fragaria vesca
-
 UDP + 2-coumaroyl beta-D-glucoside
-
 ?
UDP-glucose + 2-coumarate Rubus idaeus
-
 UDP + 2-coumaroyl beta-D-glucoside
-
 ?
UDP-glucose + 3,4-dimethoxycinnamate Fragaria x ananassa
-
 UDP + 3,4-dimethoxycinnamoyl beta-D-glucoside
-
 ?
UDP-glucose + 3,4-dimethoxycinnamate Fragaria vesca
-
 UDP + 3,4-dimethoxycinnamoyl beta-D-glucoside
-
 ?
UDP-glucose + 3,4-dimethoxycinnamate Rubus idaeus
-
 UDP + 3,4-dimethoxycinnamoyl beta-D-glucoside
-
 ?
UDP-glucose + 3-coumarate Fragaria x ananassa
-
 UDP + 3-coumaroyl beta-D-glucoside
-
 ?
UDP-glucose + 3-coumarate Fragaria vesca
-
 UDP + 3-coumaroyl beta-D-glucoside
-
 ?
UDP-glucose + 3-coumarate Rubus idaeus
-
 UDP + 3-coumaroyl beta-D-glucoside
-
 ?
UDP-glucose + 3-hydroxybenzoate Fragaria x ananassa
-
 UDP + 3-hydroxybenzoyl beta-D-glucoside
-
 ?
UDP-glucose + 3-hydroxybenzoate Fragaria vesca
-
 UDP + 3-hydroxybenzoyl beta-D-glucoside
-
 ?
UDP-glucose + 3-hydroxybenzoate Rubus idaeus
-
 UDP + 3-hydroxybenzoyl beta-D-glucoside
-
 ?
UDP-glucose + 4-coumarate Fragaria x ananassa
-
 UDP + 4-coumaroyl beta-D-glucoside
-
 ?
UDP-glucose + 4-coumarate Fragaria vesca
-
 UDP + 4-coumaroyl beta-D-glucoside
-
 ?
UDP-glucose + 4-coumarate Rubus idaeus
-
 UDP + 4-coumaroyl beta-D-glucoside
-
 ?
UDP-glucose + 4-hydroxybenzoate Fragaria x ananassa
-
 UDP + 4-hydroxybenzoyl beta-D-glucoside
-
 ?
UDP-glucose + 4-hydroxybenzoate Fragaria vesca
-
 UDP + 4-hydroxybenzoyl beta-D-glucoside
-
 ?
UDP-glucose + 4-hydroxybenzoate Rubus idaeus
-
 UDP + 4-hydroxybenzoyl beta-D-glucoside
-
 ?
UDP-glucose + caffeate Fragaria x ananassa
-
 UDP + caffeoyl beta-D-glucoside
-
 ?
UDP-glucose + caffeate Fragaria vesca
-
 UDP + caffeoyl beta-D-glucoside
-
 ?
UDP-glucose + caffeate Rubus idaeus
-
 UDP + caffeoyl beta-D-glucoside
-
 ?
UDP-glucose + ferulate Fragaria x ananassa
-
 UDP + feruloyl beta-D-glucoside
-
 ?
UDP-glucose + ferulate Fragaria vesca
-
 UDP + feruloyl beta-D-glucoside
-
 ?
UDP-glucose + ferulate Rubus idaeus
-
 UDP + feruloyl beta-D-glucoside
-
 ?
UDP-glucose + gallate Fragaria x ananassa
-
 UDP + galloyl beta-D-glucoside
-
 ?
UDP-glucose + gallate Fragaria vesca
-
 UDP + galloyl beta-D-glucoside
-
 ?
UDP-glucose + gallate Rubus idaeus
-
 UDP + galloyl beta-D-glucoside
-
 ?
UDP-glucose + gentisate Fragaria x ananassa
-
 UDP + gentisoyl beta-D-glucoside
-
 ?
UDP-glucose + gentisate Fragaria vesca
-
 UDP + gentisoyl beta-D-glucoside
-
 ?
UDP-glucose + gentisate Rubus idaeus
-
 UDP + gentisoyl beta-D-glucoside
-
 ?
UDP-glucose + protocatechuate Fragaria x ananassa
-
 UDP + protocatechuoyl beta-D-glucoside
-
 ?
UDP-glucose + protocatechuate Fragaria vesca
-
 UDP + protocatechuoyl beta-D-glucoside
-
 ?
UDP-glucose + protocatechuate Rubus idaeus
-
 UDP + protocatechuoyl beta-D-glucoside
-
 ?
UDP-glucose + salicylate Fragaria x ananassa
-
 UDP + salicoyl beta-D-glucoside
-
 ?
UDP-glucose + salicylate Fragaria vesca
-
 UDP + salicoyl beta-D-glucoside
-
 ?
UDP-glucose + salicylate Rubus idaeus
-
 UDP + salicoyl beta-D-glucoside
-
 ?
UDP-glucose + sinapate Fragaria x ananassa
-
 UDP + sinapoyl beta-D-glucoside
-
 ?
UDP-glucose + sinapate Fragaria vesca
-
 UDP + sinapoyl beta-D-glucoside
-
 ?
UDP-glucose + sinapate Rubus idaeus
-
 UDP + sinapoyl beta-D-glucoside
-
 ?
UDP-glucose + sorbate Fragaria x ananassa
-
 UDP + sorboyl beta-D-glucoside
-
 ?
UDP-glucose + sorbate Fragaria vesca
-
 UDP + sorboyl beta-D-glucoside
-
 ?
UDP-glucose + sorbate Rubus idaeus
-
 UDP + sorboyl beta-D-glucoside
-
 ?
UDP-glucose + syringate Fragaria x ananassa
-
 UDP + syringoyl beta-D-glucoside
-
 ?
UDP-glucose + syringate Fragaria vesca
-
 UDP + syringoyl beta-D-glucoside
-
 ?
UDP-glucose + syringate Rubus idaeus
-
 UDP + syringoyl beta-D-glucoside
-
 ?
UDP-glucose + trans-cinnamate Fragaria x ananassa
-
 UDP + trans-cinnamoyl beta-D-glucoside
-
 ?
UDP-glucose + trans-cinnamate Fragaria vesca
-
 UDP + trans-cinnamoyl beta-D-glucoside
-
 ?
UDP-glucose + trans-cinnamate Rubus idaeus
-
 UDP + trans-cinnamoyl beta-D-glucoside
-
 ?
UDP-glucose + vanillate Fragaria x ananassa
-
 UDP + vanilloyl beta-D-glucoside
-
 ?
UDP-glucose + vanillate Fragaria vesca
-
 UDP + vanilloyl beta-D-glucoside
-
 ?
UDP-glucose + vanillate Rubus idaeus
-
 UDP + vanilloyl beta-D-glucoside
-
 ?

Organism

Organism UniProt Comment Textmining
Fragaria vesca A0A2U9N624 cv. Yellow Wonder
-
Fragaria x ananassa Q2V6K1 i.e. Fragaria chiloensis x Fragaria virginiana, cv. Elsanta
-
Fragaria x ananassa Q66PF4 i.e. Fragaria chiloensis x Fragaria virginiana, cv. Elsanta
-
Rubus idaeus A0A2U9N6D2 cv. Tulameen
-

Purification (Commentary)

Purification (Comment) Organism
recombinant GST-tagged enzyme from Escherichia coli strain BL21(DE3) pLysS by glutathione affinity chromatography Fragaria x ananassa
recombinant GST-tagged enzyme from Escherichia coli strain BL21(DE3) pLysS by glutathione affinity chromatography Fragaria vesca
recombinant GST-tagged enzyme from Escherichia coli strain BL21(DE3) pLysS by glutathione affinity chromatography Rubus idaeus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the enzyme shows substrate promiscuity as it forms glucose esters of a variety of (hydroxyl)benzoic and (hydroxyl)cinnamic acids Fragaria x ananassa ?
-
-
additional information the enzyme shows substrate promiscuity as it forms glucose esters of a variety of (hydroxyl)benzoic and (hydroxyl)cinnamic acids. Substrate specificity, overview Fragaria x ananassa ?
-
-
additional information the enzyme shows substrate promiscuity as it forms glucose esters of a variety of (hydroxyl)benzoic and (hydroxyl)cinnamic acids. Substrate specificity, overview Fragaria vesca ?
-
-
additional information the enzyme shows substrate promiscuity as it forms glucose esters of a variety of (hydroxyl)benzoic and (hydroxyl)cinnamic acids. Substrate specificity, overview Rubus idaeus ?
-
-
UDP-glucose + 2-coumarate
-
 Fragaria x ananassa UDP + 2-coumaroyl beta-D-glucoside
-
 ?
UDP-glucose + 2-coumarate
-
 Fragaria vesca UDP + 2-coumaroyl beta-D-glucoside
-
 ?
UDP-glucose + 2-coumarate
-
 Rubus idaeus UDP + 2-coumaroyl beta-D-glucoside
-
 ?
UDP-glucose + 3,4-dimethoxycinnamate
-
 Fragaria x ananassa UDP + 3,4-dimethoxycinnamoyl beta-D-glucoside
-
 ?
UDP-glucose + 3,4-dimethoxycinnamate
-
 Fragaria vesca UDP + 3,4-dimethoxycinnamoyl beta-D-glucoside
-
 ?
UDP-glucose + 3,4-dimethoxycinnamate
-
 Rubus idaeus UDP + 3,4-dimethoxycinnamoyl beta-D-glucoside
-
 ?
UDP-glucose + 3-coumarate
-
 Fragaria x ananassa UDP + 3-coumaroyl beta-D-glucoside
-
 ?
UDP-glucose + 3-coumarate
-
 Fragaria vesca UDP + 3-coumaroyl beta-D-glucoside
-
 ?
UDP-glucose + 3-coumarate
-
 Rubus idaeus UDP + 3-coumaroyl beta-D-glucoside
-
 ?
UDP-glucose + 3-hydroxybenzoate
-
 Fragaria x ananassa UDP + 3-hydroxybenzoyl beta-D-glucoside
-
 ?
UDP-glucose + 3-hydroxybenzoate
-
 Fragaria vesca UDP + 3-hydroxybenzoyl beta-D-glucoside
-
 ?
UDP-glucose + 3-hydroxybenzoate
-
 Rubus idaeus UDP + 3-hydroxybenzoyl beta-D-glucoside
-
 ?
UDP-glucose + 4-coumarate
-
 Fragaria x ananassa UDP + 4-coumaroyl beta-D-glucoside
-
 ?
UDP-glucose + 4-coumarate
-
 Fragaria vesca UDP + 4-coumaroyl beta-D-glucoside
-
 ?
UDP-glucose + 4-coumarate
-
 Rubus idaeus UDP + 4-coumaroyl beta-D-glucoside
-
 ?
UDP-glucose + 4-hydroxybenzoate
-
 Fragaria x ananassa UDP + 4-hydroxybenzoyl beta-D-glucoside
-
 ?
UDP-glucose + 4-hydroxybenzoate
-
 Fragaria vesca UDP + 4-hydroxybenzoyl beta-D-glucoside
-
 ?
UDP-glucose + 4-hydroxybenzoate
-
 Rubus idaeus UDP + 4-hydroxybenzoyl beta-D-glucoside
-
 ?
UDP-glucose + caffeate
-
 Fragaria x ananassa UDP + caffeoyl beta-D-glucoside
-
 ?
UDP-glucose + caffeate
-
 Fragaria vesca UDP + caffeoyl beta-D-glucoside
-
 ?
UDP-glucose + caffeate
-
 Rubus idaeus UDP + caffeoyl beta-D-glucoside
-
 ?
UDP-glucose + ferulate
-
 Fragaria x ananassa UDP + feruloyl beta-D-glucoside
-
 ?
UDP-glucose + ferulate
-
 Fragaria vesca UDP + feruloyl beta-D-glucoside
-
 ?
UDP-glucose + ferulate
-
 Rubus idaeus UDP + feruloyl beta-D-glucoside
-
 ?
UDP-glucose + gallate
-
 Fragaria x ananassa UDP + galloyl beta-D-glucoside
-
 ?
UDP-glucose + gallate
-
 Fragaria vesca UDP + galloyl beta-D-glucoside
-
 ?
UDP-glucose + gallate
-
 Rubus idaeus UDP + galloyl beta-D-glucoside
-
 ?
UDP-glucose + gentisate
-
 Fragaria x ananassa UDP + gentisoyl beta-D-glucoside
-
 ?
UDP-glucose + gentisate
-
 Fragaria vesca UDP + gentisoyl beta-D-glucoside
-
 ?
UDP-glucose + gentisate
-
 Rubus idaeus UDP + gentisoyl beta-D-glucoside
-
 ?
UDP-glucose + protocatechuate
-
 Fragaria x ananassa UDP + protocatechuoyl beta-D-glucoside
-
 ?
UDP-glucose + protocatechuate
-
 Fragaria vesca UDP + protocatechuoyl beta-D-glucoside
-
 ?
UDP-glucose + protocatechuate
-
 Rubus idaeus UDP + protocatechuoyl beta-D-glucoside
-
 ?
UDP-glucose + salicylate
-
 Fragaria x ananassa UDP + salicoyl beta-D-glucoside
-
 ?
UDP-glucose + salicylate
-
 Fragaria vesca UDP + salicoyl beta-D-glucoside
-
 ?
UDP-glucose + salicylate
-
 Rubus idaeus UDP + salicoyl beta-D-glucoside
-
 ?
UDP-glucose + sinapate
-
 Fragaria x ananassa UDP + sinapoyl beta-D-glucoside
-
 ?
UDP-glucose + sinapate
-
 Fragaria vesca UDP + sinapoyl beta-D-glucoside
-
 ?
UDP-glucose + sinapate
-
 Rubus idaeus UDP + sinapoyl beta-D-glucoside
-
 ?
UDP-glucose + sorbate
-
 Fragaria x ananassa UDP + sorboyl beta-D-glucoside
-
 ?
UDP-glucose + sorbate
-
 Fragaria vesca UDP + sorboyl beta-D-glucoside
-
 ?
UDP-glucose + sorbate
-
 Rubus idaeus UDP + sorboyl beta-D-glucoside
-
 ?
UDP-glucose + syringate
-
 Fragaria x ananassa UDP + syringoyl beta-D-glucoside
-
 ?
UDP-glucose + syringate
-
 Fragaria vesca UDP + syringoyl beta-D-glucoside
-
 ?
UDP-glucose + syringate
-
 Rubus idaeus UDP + syringoyl beta-D-glucoside
-
 ?
UDP-glucose + trans-cinnamate
-
 Fragaria x ananassa UDP + trans-cinnamoyl beta-D-glucoside
-
 ?
UDP-glucose + trans-cinnamate
-
 Fragaria vesca UDP + trans-cinnamoyl beta-D-glucoside
-
 ?
UDP-glucose + trans-cinnamate
-
 Rubus idaeus UDP + trans-cinnamoyl beta-D-glucoside
-
 ?
UDP-glucose + vanillate
-
 Fragaria x ananassa UDP + vanilloyl beta-D-glucoside
-
 ?
UDP-glucose + vanillate
-
 Fragaria vesca UDP + vanilloyl beta-D-glucoside
-
 ?
UDP-glucose + vanillate
-
 Rubus idaeus UDP + vanilloyl beta-D-glucoside
-
 ?

Synonyms

Synonyms Comment Organism
FaGT2
-
 Fragaria x ananassa
FaGT5
-
 Fragaria x ananassa
FvGT2
-
 Fragaria vesca
RiGT2
-
 Rubus idaeus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Fragaria x ananassa
30
-
 assay at Fragaria vesca
30
-
 assay at Rubus idaeus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.5
-
 4-hydroxybenzoate recombinant enzyme, pH 7.0, 30°C Fragaria x ananassa
0.5
-
 syringate recombinant enzyme, pH 7.0, 30°C Fragaria x ananassa
0.7
-
 sinapate recombinant enzyme, pH 7.0, 30°C Fragaria x ananassa
0.8
-
 gallate recombinant enzyme, pH 7.0, 30°C Fragaria x ananassa
0.9
-
 vanillate recombinant enzyme, pH 7.0, 30°C Fragaria x ananassa
1.1
-
 gallate recombinant enzyme, pH 7.0, 30°C Fragaria vesca
1.2
-
 sinapate recombinant enzyme, pH 7.0, 30°C Fragaria x ananassa
1.6
-
 gallate recombinant enzyme, pH 7.0, 30°C Rubus idaeus
1.8
-
 syringate recombinant enzyme, pH 7.0, 30°C Fragaria x ananassa
2
-
 4-hydroxybenzoate recombinant enzyme, pH 7.0, 30°C Fragaria x ananassa
2.1
-
 vanillate recombinant enzyme, pH 7.0, 30°C Fragaria x ananassa
2.2
-
 UDP-glucose recombinant enzyme, pH 7.0, 30°C Fragaria vesca
3.8
-
 UDP-glucose recombinant enzyme, pH 7.0, 30°C Fragaria x ananassa
4.5
-
 UDP-glucose recombinant enzyme, pH 7.0, 30°C Fragaria x ananassa
6.6
-
 UDP-glucose recombinant enzyme, pH 7.0, 30°C Rubus idaeus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 assay at Fragaria x ananassa
7
-
 assay at Fragaria vesca
7
-
 assay at Rubus idaeus

General Information

General Information Comment Organism
physiological function the enzyme catalyzes the formation of 1-O-galloyl-beta-D-glucopyranose (beta-glucogallin) the precursor of ellagitannin biosynthesis Fragaria x ananassa
physiological function the enzyme catalyzes the formation of 1-O-galloyl-beta-D-glucopyranose (beta-glucogallin) the precursor of ellagitannin biosynthesis Fragaria vesca
physiological function the enzyme catalyzes the formation of 1-O-galloyl-beta-D-glucopyranose (beta-glucogallin) the precursor of ellagitannin biosynthesis Rubus idaeus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
4.74
-
 gallate recombinant enzyme, pH 7.0, 30°C Fragaria vesca
5.77
-
 vanillate recombinant enzyme, pH 7.0, 30°C Fragaria x ananassa
6.35
-
 4-hydroxybenzoate recombinant enzyme, pH 7.0, 30°C Fragaria x ananassa
6.87
-
 syringate recombinant enzyme, pH 7.0, 30°C Fragaria x ananassa
6.92
-
 UDP-glucose recombinant enzyme, pH 7.0, 30°C Fragaria vesca
8.33
-
 gallate recombinant enzyme, pH 7.0, 30°C Fragaria x ananassa
8.51
-
 UDP-glucose recombinant enzyme, pH 7.0, 30°C Fragaria x ananassa
9.57
-
 vanillate recombinant enzyme, pH 7.0, 30°C Fragaria x ananassa
9.66
-
 syringate recombinant enzyme, pH 7.0, 30°C Fragaria x ananassa
9.71
-
 4-hydroxybenzoate recombinant enzyme, pH 7.0, 30°C Fragaria x ananassa
10.05
-
 UDP-glucose recombinant enzyme, pH 7.0, 30°C Fragaria x ananassa
15.56
-
 sinapate recombinant enzyme, pH 7.0, 30°C Fragaria x ananassa
15.83
-
 UDP-glucose recombinant enzyme, pH 7.0, 30°C Rubus idaeus
20
-
 gallate recombinant enzyme, pH 7.0, 30°C Rubus idaeus
34.29
-
 sinapate recombinant enzyme, pH 7.0, 30°C Fragaria x ananassa