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Literature summary for 2.4.1.178 extracted from
- Prunasin biosynthesis by cell-free extracts from black cherry (Prunus serotina Ehrh.) fruits and leaves (1983), Z. Naturforsch. C, 38, 369-374.
No PubMed abstract available
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.32 | - |
UDP-glucose | assay at pH 7.3 in the presence of 20 mM of (R,S)-mandelonitrile. The enzyme exclusively utilizes the (R)-enantiomer | Prunus serotina |  |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| UDP-glucose + (R,S)-mandelonitrile | Prunus serotina | - |
UDP + (R)-prunasin | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Prunus serotina | - |
wild black cherry | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| method that includes chromatography on Sephadex G-25 | Prunus serotina |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.001 | - |
mandelic acid | Prunus serotina |
| 0.002 | - |
benzyl alcohol | Prunus serotina |
| 0.0025 | - |
mandelamide | Prunus serotina |
| 0.012 | - |
S)-mandelonitrile | Prunus serotina |
| 0.018 | - |
benzoic acid | Prunus serotina |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, 10% glycerol, 72% of 2 days crude extract stable for several weeks | Prunus serotina |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| UDP-glucose + (R,S)-mandelonitrile | - |
Prunus serotina | UDP + (R)-prunasin | - |
? | |
| UDP-glucose + benzoic acid | - |
Prunus serotina | UDP + benzoyl-beta-D-glucoside | - |
? | |
| UDP-glucose + benzyl alcohol | - |
Prunus serotina | UDP + benzyl-beta-D-glucoside | - |
? | |
| UDP-glucose + mandelamide | - |
Prunus serotina | ? | - |
? | |
| UDP-glucose + mandelic acid | - |
Prunus serotina | ? | - |
? | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 8 | highest rates are realized in Tris-HCl buffer at pH 7.5 | Prunus serotina |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5.8 | 9.3 | about 50% of activity maximum | Prunus serotina |
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