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Literature summary for 2.4.1.18 extracted from
- Major isoforms of starch branching enzymes in premature seeds of kidney bean (Phaseolus vulgaris L.) (2001), Biosci. Biotechnol. Biochem., 65, 1141-1148.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| citrate | activation | Phaseolus vulgaris |  |
| citrate | 3.3fold activation at 0.3 M, enzyme form KBE1 and KBE2 | Phaseolus vulgaris | |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| CaCl2 | 10 mM, 50% inhibition. 100 mM, complete inactivation | Phaseolus vulgaris | |
| CuSO4 | 1 mM, complete inactivation | Phaseolus vulgaris | |
| HgCl2 | 1 mM, complete inactivation | Phaseolus vulgaris | |
| MgCl2 | 1 mM, complete inactivation | Phaseolus vulgaris | |
| MnCl2 | 1 mM, complete inactivation | Phaseolus vulgaris | |
| ZnCl2 | 1 mM, complete inactivation | Phaseolus vulgaris | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | Km-value for amylose, enzyme form KBE1: 1.27 mg/ml. Km-value for amylose, enzyme form KBE2: 0.74 mg/ml | Phaseolus vulgaris |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 77000 | - |
1 * 77000, enzyme KBE2, SDS-PAGE | Phaseolus vulgaris |
| 80000 | - |
1 * 80000, enzyme KBE1, SDS-PAGE | Phaseolus vulgaris |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Phaseolus vulgaris | - |
L. Toramame | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Phaseolus vulgaris |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| seed | developing seeds contain several isoforms. The major forms are KBE1 and KBE2 | Phaseolus vulgaris | - |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 77000, enzyme KBE2, SDS-PAGE | Phaseolus vulgaris |
| monomer | 1 * 80000, enzyme KBE1, SDS-PAGE | Phaseolus vulgaris |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | 30 | - |
Phaseolus vulgaris |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
pH 8.0, 15 min, stable up to, enzyme form KBE1 | Phaseolus vulgaris |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
- |
Phaseolus vulgaris |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 9.5 | stable | Phaseolus vulgaris |
| 7 | 9.5 | 4°C, 16 h, less than 10% loss of activity, enzyme form KBE1 and KBE2 | Phaseolus vulgaris |
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