Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli Bl21-CodonPlus(DE3)-RIL and pET21a(+) | Thermococcus kodakarensis |
Protein Variants | Comment | Organism |
---|---|---|
additional information | recombinant TK1436 protein (amino acids [aa] 1 to 675) and a deletion derivative devoid of the C-terminal two-copy helix-hairpin-helix (HhH)2 motif (TK1436deltaH, aa 1 to 562) | Thermococcus kodakarensis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
78550 | - |
calculated molecular mass of the TK1436 protein | Thermococcus kodakarensis |
558000 | - |
TK1436deltaH protein, gel filtration on a Superdex 200 HR 10/30 column | Thermococcus kodakarensis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermococcus kodakarensis | Q5JDJ7 | TK1436 encodes a protein of 675 aa; hyperthermophilic archaeon | - |
Purification (Comment) | Organism |
---|---|
anion exchange column and hydrophobic column | Thermococcus kodakarensis |
Storage Stability | Organism |
---|---|
the TK1436 protein shows rapid degradation within 2 to 3 days of storage at 4°C, while the TK1436deltaH protein is stable unter the same conditions | Thermococcus kodakarensis |
Synonyms | Comment | Organism |
---|---|---|
TK1436 | encodes a branching enzyme from Thermococcus kodakaraensis belonging to the GH-57 family. The TK1436 protein contains neither alpha-galactosidase nor pullulanase activity. | Thermococcus kodakarensis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
determined by iodine-staining assay | Thermococcus kodakarensis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
90 | - |
the protein is stable up to 90°C, the activity decreases gradually during the incubation at 100°C | Thermococcus kodakarensis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
determined by iodine-staining assay | Thermococcus kodakarensis |