Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.4.1.18 extracted from

  • Kim, E.; Ryu, S.; Bae, H.; Huong, N.; Lee, S.
    Biochemical characterisation of a glycogen branching enzyme from Streptococcus mutans: Enzymatic modification of starch (2008), Food Chem., 110, 979-984.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli BL21 Streptococcus mutans

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
74000
-
SDS-PAGE Streptococcus mutans

Organism

Organism UniProt Comment Textmining
Streptococcus mutans Q8DT52
-
-

Purification (Commentary)

Purification (Comment) Organism
His-tag affinity column chromatography Streptococcus mutans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
amylose assay at pH 5.0, 37°C Streptococcus mutans ?
-
?
starch assay at pH 5.0, 37°C Streptococcus mutans ?
-
?

Synonyms

Synonyms Comment Organism
alpha-1,4-glucan branching enzyme
-
Streptococcus mutans
glycogen branching enzyme
-
Streptococcus mutans
SmGBE
-
Streptococcus mutans

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Streptococcus mutans

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
above 40°C enzyme inactivated after incubation time of 1 h Streptococcus mutans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
-
Streptococcus mutans

pH Range

pH Minimum pH Maximum Comment Organism
4.5 6
-
Streptococcus mutans

pH Stability

pH Stability pH Stability Maximum Comment Organism
4 8 relatively stable during 1 h of incubation Streptococcus mutans