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Literature summary for 2.4.1.18 extracted from

  • Brust, H.; Lehmann, T.; DHulst, C.; Fettke, J.
    Analysis of the functional interaction of Arabidopsis starch synthase and branching enzyme isoforms reveals that the cooperative action of SSI and BEs results in glucans with polymodal chain length distribution similar to amylopectin (2014), PLoS ONE, 9, e102364.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.4.1.18

Cloned(Commentary)

Cloned (Comment) Organism
recombinant expression of His-tagged isozymes rBE2 and rBE3 in Escherichia coli strain BL21(DE3) and in strain DELTAglgCAP with BL21(DE3) background Arabidopsis thaliana

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana
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-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged isozymes rBE2 and rBE3 from Escherichia coli strains by nickel affinity chromatography Arabidopsis thaliana

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
 Arabidopsis thaliana
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information Starch synthase I activity in native PAGE without addition of glucans is dependent on at least one of the two branching enzyme isoforms active in Arabidopsis leaves. This interaction is most likely not based on a physical association of the enzymes. Chain length distribution patterns of the formed glucans are irrespective of enzyme isoforms origin and still independent of assay conditions. All starch synthase isoforms I-IV are able to interact with branching enzymes and form branched glucans, product analysis, overview Arabidopsis thaliana ?
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 ?

Synonyms

Synonyms Comment Organism
SBE
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 Arabidopsis thaliana
starch branching enzyme
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 Arabidopsis thaliana

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 assay at Arabidopsis thaliana

General Information

General Information Comment Organism
metabolism starch synthase and branching enzyme establish the two glycosidic linkages existing in starch. Both enzymes exist as several isoforms. Starch synthase I activity in native PAGE without addition of glucans is dependent on at least one of the two branching enzyme isoforms active in Arabidopsis leaves. This interaction is most likely not based on a physical association of the enzymes. All starch synthase isoforms I-IV are able to interact with branching enzymes and form branched glucans Arabidopsis thaliana